包裹BSA的线状银为基底探讨小檗碱的拉曼光谱

Surface Enhanced Raman Spectroscopy Study of Berberine Based on BSA-Coated Silver Nanowires

通过拉曼光谱技术测定了液体小檗碱(Berberine)的常规拉曼光谱(NRS)及表面增强拉曼光谱(SERS),归属了拉曼特征峰,并与固体小檗碱的NRS进行对比,发现特征峰位基本没变.研究小檗碱与纳米银(Ag-Berberine)、包裹牛血清白蛋白(BSA)的线状银(Ag@BSA-Berberine)的拉曼光谱,分析了线性纳米银(Ag)及包裹BSA的线状银(Ag@BSA)对小檗碱的吸附模型.结果显示,线性纳米银对小檗碱、牛血清白蛋白有明显的拉曼增强效应,增强效应主要在线性纳米银与氨基酸中N的孤电子对、甲氧基中的氧、苯环中的π电子及异喹啉中的N发生了吸附作用.对比Ag@BSA-Berberine和Ag-Berberine的特征峰,加了BSA后的拉曼光谱有明显的减弱,但峰位并没有移动,但是有BSA修饰的纳米银的小檗碱拉曼光谱有较高的稳定性,利于小檗碱的进一步研究.

The normal Raman spectroscopy(NRS)and surface enhanced Raman spectroscopy(SERS)of the solutions for berberine were reported,and the vibrational peaks were assigned.SERS of solutions and NRS of solids for berberine were compared.The results showed that the positions of characteristic peaks were not changed,but the strengthen of peaks were enhanced relatively.The study of the SERS spectra of berberine with silver nanowires(Ag-Berberine)and that with bovine serum albumin(BSA)-coated silver nanowires(Ag@BSA-Berberine)were analyzed the influence on their systems and the adsorption way of silver nanowires.The results indicated that the SERS spectra of Ag-Berberine increased obviously,which developed a convenient way to analyze the characteristic peaks of bernerine.Enanced Raman spectra expressed that Ag nanowires were linked to N atom of amino acid,O atom of methoxyl,electron of benzene ring and N atom of isoquinoline.Compared the SERS spectra of Ag-Berberine and Ag@BSA-Berberine,it displayed that the SERS spectra of Ag@BSA-Berberine was lower than Ag-Berberine,but it was represented highly stabilization and peaks of Berberine were not maced,which provided important information for further study.