火把花根预处理对油酸致大鼠急性肺损伤的作用研究

The Role of Colquhounia Root Tablet against Acute Lung Injury Oleic Acid Pretreated in Rats

目的探讨火把花根对油酸所致急性肺损伤大鼠的保护作用。方法 SD大鼠45只随机分成正常对照组(N组)、急性肺损伤组(ALI组)和火把花组(C组),每组各15只。正常对照组:每次2mL生理盐水,每天2次,连续灌胃10d,第10天灌胃后1h尾静脉注射生理盐水0.04mL·kg-1;ALI组:生理盐水灌胃同正常对照组,第10天灌胃后1h尾静脉注射油酸(0.04mL·kg-1),复制大鼠ALI模型;火把花根组:火把花根片灌胃10d[600mg·(kg·d)-1],分每天2次进行,第10天灌胃后1h尾静脉注射油酸(0.04mL·kg-1)。各组注射油酸后4h采集肺组织,测定肺湿/干重比(W/D);采集静脉血、支气管肺泡灌洗液,测定肺通透指数(LPI);肺组织切片HE染色行肺损伤评分(LIS)。结果肉眼观察正常对照组肺组织未见明显异常;ALI组肿胀明显;火把花根组轻度肿胀。光镜下正常对照组未见明显病理改变;ALI组肺泡壁欠完整,肺泡腔内见大量炎症细胞浸润,红细胞渗出,肺泡间质明显水肿;火把花根组改变较ALI组明显减轻。ALI组LIS较正常对照组明显升高(P<0.01),火把花根组LIS明显低于ALI组(P<0.01),但仍高于正常对照组(P<0.05)。ALI组W/D和LPI分别较正常对照组、火把花根组升高(P<0.05),火把花根组W/D高于正常对照组(P<0.01),但火把花根组LPI与正常对照组无明显差异。结论火把花根对油酸所致大鼠急性肺损伤有一定的保护作用。

Objective To explore the protective effects of colquhounia root on rats with acute lung injury induced by oleic acid. Methods 45 healthy SD rats were randomly divided into three groups: the control group( N group),the ALI group and the colquhounia root treated group( C group) so 15 rats in each group. In N group,sodium chloride 2mL were instilled into the stomach of each rat for ten consecutive days before intravenous injection of sodium chloride 0. 04mL·kg-1. In ALI group,sodium chloride 2mL were instilled into the stomach of each rat for ten consecutive days until oleic acid 0. 04mL·kg-1was injected into trail vein to establish ALI models,and in C group,colquhounia root talet with 600mg·( kg·d)-1were instilled into the stomach of each rat for ten consecutive days before intravenous injection of same dose oleic acid with the ALI group. After 4h of establishing model,all animals were sacrificed to collect lung tissue of rats to test lung tissue wet / dry weight ratio( W / D),collect rat blood serum,bronchoalveolar lavage fluid to test lung permeability index( LPI). Lung tissue was fixed-embedded to do hematoxylin-eosin( HE) staining to test lung injury score( LIS). Results Through general observation,the lung tissue was normal in N group. In ALI group, the lung tissue swelled,and there were widespread bleeding points on the surface. In C group,lung tissue swelled slightly. Under the microscope,alveolar epithelium was integrity,and there was no significant pathological change in alveolar spaces and pulmonary interstitium in N group. In ALI group,the alveolar wall was not complete. There were lots of inflammatory cells infiltration,red blood cell exudation and alveolar interstitial edema. Change in C group was not serious. The LIS of ALI group was significantly increased than that of N group( P < 0. 01). The LIS of C group was significantly lower than that of ALI group( P < 0. 01),but still higher than that of N group( P < 0. 05). In ALI group,W / D and LPI were respectively significantly increased than that of N group and C group( P < 0. 05). In C group,W / D was higher than that of N group( P < 0. 01), but there was no significant difference between that of C group and N group. Conclusion The colquhounia root can protect rats from oleic acid induced acute lung injury.