不同治法中药药物血清对TNF-α刺激下动脉内皮细胞保护作用的研究

Protective Effect of Different Principle Containing Serum of the Chinese Traditional Medicine on VECs Injuried by TNF-α

目的探讨不同治法中药药物血清,对体外培养TNF-α损伤动脉内皮细胞结构及细胞活力的影响。方法通过光镜及电境观察清法、软坚法、活血法等不同治法中药药物血清对体外培养TNF-α损伤的动脉血管内皮细胞光镜下形态及超微结构的影响。通过MTT法测定血管内皮细胞活力。结果光镜及电镜下体外培养TNF-α刺激下的动脉内皮细胞结构有明显损伤。而加入不同治法中药药物血清的各组,TNF-α所致动脉内皮细胞结构损伤均有不同程度的减轻。MTT结果显示,用TNF-α处理过后,体外培养的内皮细胞的增殖明显受到抑制(P<0.01),经过与不同中药药物血清共同孵育后,细胞增殖率均明显上升(P<0.01)。其中以清法组、软坚组作用最明显。结论不同治法中药药物血清对体外培养TNF-α刺激下的损伤动脉内皮细胞均具有保护作用。

Objective To explore the effects of different traditional Chinese medicines serum on cultured arterial endothelial cell structure and cell viability which were damaged by TNF-α. Methods The effects of different therapies and medicine serum on artery endothelial cell gross morphology and ultrastructure injuried by TNF-α were observed by light microscope and electron border,and the endothelial cell viability were measured by MTT assay. Results The arterial endothelial cell structures stimulated by TNF-α were found heavily damaged by light microscopy and electron. While the other groups,added with different Chinese medicines serum,the situation were better despite of TNF-α stimulation. According to MTT assay results,the endothelial cell proliferation was significantly inhibited when stimulated by TNF-α( P < 0. 01). However,when the groups were incubated with different Chinese medicines serum,the cell proliferation was significantly increased( P <0.01),of which,the Qingfa and Ruanjian groups were the most significant ones. Conclusion Different Chinese medicine serum therapies have protective effects on arterial endothelial cells injuryied by TNF-α,of which the Qingfa and Ruanjian groups were the most significant ones.