摘要
目的探讨白细胞介素-10(interleukin10,IL-10)基因启动子-592 A/C多态性与宁夏地区汉族女性复发性自然流产(recurrent spontaneous abortion,RSA)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对228例反复自然流产患者和213例健康对照的IL-10基因启动子-592A/C多态性位点进行检测。结果 IL-10基因SNP-592 A/C的AA、AC、CC基因型在病例组中的分布频率分别为32.89%、51.32%和15.79%,在对照组中的分布频率分别为47.89%、44.13%和7.98%,两组的基因型分布频率差异有统计学意义(P<0.05)。IL-10基因SNP-592位点A、C等位基因在病例组中的分布频率分别为58.55%和41.45%,在对照组中的分布频率分别为69.95%和30.05%。两组的等位基因分布频率差异亦有统计学意义(P<0.05)。结论 IL-10基因启动子-592A/C位点可能与宁夏地区汉族女性复发性自然流产的遗传易感性有关。
Objective To investigate the relationship between polymorphism of interleukin- 10( IL- 10)gene promoter- 592 A / C and recurrent spontaneous abortion. Methods The gene IL- 10- 592 A / C polymorphisms was examined by the polymerase chain reaction- restriction fragment length polymorphism( PCR-RFLP) methods in 228 recurrent spontaneous abortion patients and 213 normal pregnancy women. Results IL- 10 gene at the promoter- 592 A / C the frequencies of AA,AC and CC genotype were 32. 89%,51. 32%and 15. 79% in patients with recurrent spontaneous abortion,while 47. 89%,44. 13% and 7. 98% in healthy controls. A significant difference was seen in two groups( P < 0. 05). IL- 10 gene at the promoter- 592 A /C the allele gene frequency of A and C were 58. 55% and 41. 45% in patients with recurrent spontaneous abortion,while 69. 95% and 30. 05% in healthy controls. A significant difference was seen in two groups too( P < 0. 05). Conclusion The polymorphisms of interleukin- 10( IL-10) gene at the promoter- 592( A / C) was related potentially with recurrent spontaneous abortion in Ningxia female.
出处
《宁夏医科大学学报》
2014年第10期1073-1075,1081,1064,共5页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(NZ13155)
宁夏科技攻关项目(200932)
