胰岛素样生长因子-2在胶质瘤中表达的意义

The Expression of Insulin-like Growth Factor-2 in Tissues of Human Gliomas

目的探究胰岛素样生长因子-2(IGF2)在胶质瘤中表达的意义。方法运用HE染色、免疫组化、FQ-RT-PCR的实验方法,检测不同级别胶质瘤中IGF2的表达水平。结果 HE染色示高、低级别胶质瘤组织在细胞排列形式及细胞形态上存在差异;免疫组化示IGF2在高、低级别胶质瘤组织中均有表达,且高级别组高于低级别组(P<0.05);FQ-RT-PCR可见IGF2的相对表达量在高级别组(1.19±1.40)高于低级别组(0.06±0.02)(P<0.05)。结论 IGF2的表达随着胶质瘤病理级别的增高而增加,在胶质瘤的发生发展中发挥一定作用。

Objective To explore the significance of the expression of Insulin- like growth factor- 2( IGF2)in tumor tissues of gliomas patients. Methods The expressional level of IGF2 in gliomas tissue was detected by HE and immunohistochemical while the relative quantitative expression of IGF2 was determined by the method of FQ- RT- PCR. Results The results showedthat IGF2 expressed both in high and low grade gliomas tissue,and the level of IGF2 expressed in tumor of patients with high- grade group was significantly higher than that in the low- grade group by IHC( P < 0. 05). The level of IGF2 presented in serum as follows:high- grade group( 1. 19 ± 1. 40) was significantly higher than that in low- grade group( 0. 06 ± 0. 02).Conclusion IGF2 plays an important role in the production and procession of gliomas,and it is associated with the classification of gliomas.