HBV血清学标志物特殊组合模式与HBV-DNA检测结果分析

The Relationship between Special HBV-M and Special Models of HBV-DNA

目的分析乙型肝炎标志物(HBV-M)特殊组合模式的HBV-DNA水平,探讨不同HBV-M组合模式与病毒复制的相关性及其临床意义。方法分别利用化学发光法和荧光定量PCR法定量检测205例乙肝患者血清学标志物(HBs Ag、HBs Ab、HBe Ag、HBe Ab、HBc Ab分别作为1、2、3、4、5项)及HBV-DNA,分析乙型病毒血清标志物的各种特殊组合模式。结果 4种阳性模式组的HBs Ag差异有统计学意义(χ2=53.65,P<0.001);1、2、3、5阳性模式组高于其他阳性模式组(P<0.0083);4种阳性模式组的HBV-DNA病毒载量差异有统计学意义(χ2=64.19,P<0.001),1、2、3、5阳性模式高于1、2、4、5阳性模式组(P<0.0083)。1、3、4、5阳性模式与1、3阳性模式的HBe Ag表达量及HBV-DNA病毒载量差异有统计学意义(P<0.001)。结论HBV血清标志物中抗原和抗体共存的特殊组合模式具有多样性,正确合理地分析HBV-M组合类型结合HBV-DNA的定量检测有助于指导临床治疗。

Objective To investigate the relationship between some special models of HBVM and HBV- DNA quantification through quantity analysis. Methods HBV- M for 205 samples obtained from routine work in clinical laboratory were detected by time- resolution immunofluorescence. HBV- DNA quantity of those samples were tested with the real time fluorescent quantitative PCR instrument. Results The positive rate of HBs Ag in four coexistence model were significantly different( χ2= 53. 65,P < 0. 001). Coexistence of 1,2,3,5,the positive rate of HBs Ag was significantly higher than that in other models( P < 0. 0083). HBV- DNA contents in four coexistence model were significantly different( χ2= 64. 12,P < 0. 001). HBV- DNA contents in coexistence of1,2,3,5 were significantly higher than those in coexistence of 1,2,4,5( P < 0. 0083). Coexistence of 1,3,4,5,the positive rate of HBe Ag and HBV- DNA content were significantly different from that in coexistence of 1,3( P <0. 001). Conclusion The results demonstrate the multiformity of HBV- M coexistence. Correlation analysis of different models detection of HBV serum markers and a real- time test of HBV- DNA are helpful to clinical diagnosis,treatment and monitoring for the state of HBV infection and its complication.