乳腺癌组织中耐药相关基因MDR1、BCRP和LRP表达的研究

Expression of Drug-resistance Related Genes in Breast Carcinoma

目的探讨乳腺癌组织中多药耐药基因(MDR1)、乳腺癌耐药蛋白(BCRP)和肺耐药蛋白(LRP)基因的表达情况,分析它们与临床病理特征及与雌激素(ER)、孕激素(PR)和原癌基因C-erb B-2之间的相关性。方法采用RT-PCR方法检测42例乳腺癌组织及癌旁组织中MDR1、BCRP和LRP的mRNA的表达情况。结果 1MDR1、BCRP和LRP的mRNA在乳腺癌组织中的阳性表达率和相对表达量均高于癌旁组织(P均<0.05);2 MDR1 mRNA表达与乳腺癌临床分期及腋窝淋巴结转移呈正相关(r=0.369、0.398,P<0.05或<0.01);BCRP mRNA表达与腋窝淋巴结转移呈正相关(r=0.355,P<0.01),与临床分期无相关性(P>0.05);LRP mRNA表达与临床分期、腋窝淋巴结转移均无相关性(P>0.05);3MDR1 mRNA表达与C-erb B-2表达呈正相关(r=0.311,P<0.05),BCRP mRNA、LRP mRNA与C-erb B-2表达均无相关性(P>0.05)。结论多药耐药基因MDR1和BCRP的mRNA表达水平可能作为预测乳腺癌生物学行为的参考指标。

Objective To investigate the expression of the Multidrug resistance gene 1,breast cancer resistance protein and lung cancer resistance protein gene in breast cancer tissues,and to analyze the relationship between these three genes and clinical pathological features and ER、PR and C- erb B- 2. Methods Expression of MDR1 mRNA,BCRP mRNA and LRP mRNA in tumor tissues and the adjacent tissues of 42 cases with breast cancer by RT- PCR. Results 1The positive expression rate and relative coefficient of MDR1 mRNA,BCRP mRNA and LRP mRNA in breast cancer tissues were significantly higher than those of the adjacent tissues( P < 0. 05); 2The expression of MDR1 mRNA was significantly positively correlated with clinical stage and axillary lymph node metastasis of breast cancer( r = 0. 369,0. 398)( P < 0. 05,P < 0. 01). The expression of BCRP mRNA was significantly positively correlated with axillary lymph node metastasis( r = 0. 355)( P < 0. 01),and no correlation with clinical stage( P > 0. 05). The expression of LRP mRNA was not significantly correlated with clinical stage and axillary lymph node metastasis( P > 0. 05). 3 The Expression of MDR1 mRNA was significantly positively correlated with the expression of C- erb B- 2( r = 0. 311)( P <0. 05). Conclusion The expression of MDR1 mRNA and BCRP mRNA may predict the biological behavior of breast cancer.