枸杞多糖对脑出血模型大鼠继发性脑损伤的影响

The Effect of Lycium Barbarum Polysaccharides on Secondary Brain Injury in Intracerebral Hemorrhage Rats

目的探讨枸杞多糖(lycium barbarum bolysaccharides,LBP)对脑出血(intracerebral hemorrhage,ICH)模型大鼠继发性脑损伤的影响。方法 SD大鼠90只,随机分为假手术组、模型组、LBP低剂量组、LBP中剂量组、LBP高剂量组、尼莫地平组,每组15只;术前15日,LBP低剂量、LBP中剂量、LBP高剂量组分别给予LBP50、100、200mg·kg^(-1)灌胃,尼莫地平组给予尼莫地平10mg·kg^(-1)灌胃,假手术组和模型组给予等量的生理盐水,每日1次;灌胃结束后,采用自体血尾状核注射法制作大鼠脑出血模型;在脑出血后4、8、12、24和48h分别对各组大鼠进行神经功能评分;评分结束后断头取脑,检测各组大鼠脑组织水肿体积、脑含水量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;HE染色后观察脑出血区及其周围脑组织的神经细胞、胶质细胞等的大小、形态。结果与假手术组比较,其他各组大鼠神经功能评分较高、脑组织水肿体积较大、脑含水量较高、SOD活性较低、MDA含量较多(P<0.05或P<0.01)。与模型组比较,LBP各组大鼠神经功能评分仅部分时点减低(P<0.05),脑组织水肿体积减小、脑含水量降低、SOD活性增高、MDA含量降低(P<0.05或P<0.01)。假手术组HE染色结果未见明显异常,模型组与各给药组HE染色结果均显示脑出血区均有血凝块形成,出血区周围脑组织有不同程度的破坏,并可见排列紊乱的神经细胞与神经胶质细胞。结论预防性应用枸杞多糖能够减轻脑出血后继发性脑损伤,对脑出血后脑组织起到一定的保护作用,对预防脑出血有积极的意义。

Objective To explore the effect of Lycium Barbarum Polysaccharides( LBP) on the secondary brain injury in rat models of Intracerebral hemorrhage( ICH). Methods 90 SD rats were randomly divided into sham group,model group,LBP low dose group,LBP moderate dose group,LBP high dose group and nimodipine group. Preoperatively 15 th day,rats of LBP low dose group,LBP moderate dose group,LBP high dose group,was given LBP 50,100,200 mg · kg^(-1)gavage,respectively while rats of nimodipine group was given nimodipine 40 mg ·kg^(-1)gavage and rats in sham group and model group were given the same amount of saline,1 time/day. After gavage,rat ICH model was established using autologous blood injection caudate nucleus. The neurological function score grading of rats was carried out. the brain edema volume,brain water content,SOD activity and MDA content were detected in brain tissue of rats.the cell size and morphology werebserved after HE staining. Results Compared with sham group,the neurological function score of rats was higher,brain edema volume was larger,brain water content was taller,SOD activity was lower and MDA content was higher in other groups( P < 0. 05 or P < 0. 01). Compared with model group,neurological function scores at part timepoint in LBP groups were reduced( P < 0. 05),brain edema volume was alleviated,brain water content was reduced,SOD activity increased and MDA content decreased significantly( P < 0. 05 or P < 0. 01). The HE staining of sham group showed no significant abnormalities,but the HE staining in other groups showed: blood clot formed in the ICH region,brain tissue around the ICH region had different degree of damage,and nerve cells and gliocyte cells disordered arrangement. Conclusion Prophylactic use of LBP can reduce the secondary brain injury of ICH. It has a protective effect on ICH and has a positive significance in prevention against ICH.