宁夏回族与汉族2型糖尿病患者血浆蛋白表达差异分析

The Expressions of Different Proteins in Plasma of the Hui and Han Population with Type 2 Diabetes Mellitus in Ningxia

目的分析宁夏回、汉族2型糖尿病(type2 diabetes mellitus,T2DM)患者血浆的蛋白表达差异。方法收集T2DM汉族组(n=5)、T2DM回族组(n=5)和正常对照组(n=4)的血浆标本,采用ALB/Ig G吸附柱去除高丰度蛋白,液相色谱-质谱技术进行分离、筛选,应用Proteome Discoverer软件和NCBI Human-Ref Seq(人参考序列库)数据库进行蛋白表达的信息分析。结果以蛋白1.5倍变化为标准,对235个蛋白的表达结果分析显示:T2DM汉族组主要上调蛋白有7个,下调蛋白10个;T2DM回族组主要上调蛋白9个,下调蛋白6个。以T2DM回、汉族组间差异蛋白的2倍变化为标准,发现以下有显著变化的蛋白:T2DM汉族组上调蛋白为脂多糖结合蛋白(LBP)、葡萄糖磷酸变位酶(PGM1),下调蛋白为角蛋白1、2、14和10(KRT1、KRT2、KRT14、KRT10)、热休克蛋白90α(HSP90α);T2DM回族组上调蛋白为载脂蛋白C2、C3(APOC2、APOC3),下调蛋白为二硫化物异构酶A3(PDIA3)和血小板蛋白(THBS1)。结论宁夏T2DM患者汉族组、回族组血浆蛋白表达有差异。

Objective To screen and analyze the expression of different proteins in plasma of the Hui and Han population with type 2 diabetes mellitus in Ningxia. Methods The enrolled subjects into three groups: normal control group( n = 4),the Han group with type 2 diabetes mellitus( n = 5) and the Hui group with type 2diabetes mellitus( n = 5). ALB / Ig G adsorption was used to remove high- abundance proteins while LC / MS was used to separate and screen proteins,The expression of different proteins were analyzed by Discoverer Software and NCBI Human- Ref Se database. Results Taken 1. 5 times changes in proteins expression as the standard,the results showed that the expressions of some kinds of proteins were significantly changed including LBP,PGM1 which were up- regulated and KRT1,KRT2,KRT14,KRT10,HSP90α which were down- regulated with significant difference in type 2 diabetes mellitus Han group. In addition,APOC2,APOC3 were up- regulated and PDIA3,THBS1 were down- regulated with significant difference in type 2 diabetes mellitus Hui group. Conclusion There were difference in the expression of plasma proteins in the Hui and Han group with type 2 diabetes mellitus in Ningxia.