携带HGF基因的脐血间充质干细胞异体移植对烧伤后大鼠心肌损伤的影响

Effect of Ad-HGF Modified Umbilical Cord Mesenchymal Stem Cells on Myocardial Injury in Burn Rats

目的评估携带腺病毒介导的肝细胞生长因子基因(Ad-HGF)的脐血间充质干细胞异体移植对烧伤后大鼠损伤的心肌的影响。方法利用密度梯度法分离脐血干细胞,并培养传代。将构建的Ad-HGF转染脐血干细胞(MOI=100)。60只大鼠(雌雄不限)建立深二度烧伤模型并分为生理盐水对照组(A组,n=12)、脐血间充质干细胞治疗组(B组,n=24)、Ad-HGF修饰的脐血间充质干细胞治疗组(C组,n=24),将不同治疗药物经尾静脉注射于不同组别大鼠体内,伤后12、24、48和72 h检测血浆心肌肌钙蛋白I(c Tn I)、心肌肌酸激酶(CK)、心肌肌酸激酶同工酶(CK-MB)以及心钠素(BNP)含量。结果烧伤后12h A、B、C组动物血浆c Tn I、CK、CK-MB和BNP均明显升高,24h后CK、CK-MB均下降,48h后c Tn I和BNP逐渐下降。各组间同时间点比较,C组各检测指标均较A、B组明显降低(P<0.05或<0.01)。结论携带HGF基因的脐血间充质干细胞异体移植对烧伤后大鼠损伤的心肌具有保护作用,为临床烧伤治疗提供了一个新思路。

Objective To explore effect of Ad- HGF modified umbilical cord mesenchymal stem cells(UCMSCs)on myocardial injury in burn rats. Methods UCMSCs were collected,cultured and transfected with Ad- HGF. 60 Wistar rats were randomized into 3 groups: UCMSCs treatment(group A,n=24),transplantation of Ad- HGF modified UCMSCs(group B,n=24)and saline control(group C,n=12). According to different groups,drugs were injected though the tail vein in burn rats. On post burn 12,24,48 and 72 hours,c Tn I,CK,CK-MB and BNP were anglyzed. Results At the 12 th hour after burn,c Tn I,CK,CK-MB and BNP were significantly increased,and by 24 thh,CK and CK-MB significantly decreased,after 48 thh,c Tn I and BNP decreased gradually. During the observation time,the index of B group had statistical significance compared with the other groups. Conclusion Ad- HGF modified UCMSCs has postive effect on the myocardial injury in burn rats,providing a new idea for the clinical job.