猪伪狂犬病蜂胶疫苗和油佐剂疫苗对小白鼠注射部位损伤的病理组织学比较

Histopathologic Comparison on the Effect of PR Inactivated Propolis Vaccine and Oil Vaccine to the Injection Site of Mice

用猪伪狂犬病 g E/ g I基因缺失蜂胶灭活疫苗和油佐剂疫苗分别腿肌注射免疫实验动物小白鼠 ,分别于免疫后 2 ,7,1 4 d和 3 5d剖杀 ,取注射部位的肌肉组织 ,常规石蜡包埋切片观察。结果表明 ,蜂胶苗注射后立即吸收 ,局部不留痕迹 ,感官与对照相比无任何差异。免疫 2 d注射局部显微变化轻微 ,7d后肌纤维间隙发现吞噬黄色蜂胶疫苗的细胞 ,1 4 d此种细胞更多 ,3 5d汇聚成片 ;油苗注射后在 3 5d的试验期内一直未能全部吸收。免疫 2 d见肌纤维断裂、肿胀、坏死 ,大量嗜中性粒细胞浸润 ,肌纤维间隙广泛分布油囊 ,7d时局部炎症仍很严重 ,1 4 d表现炎症转归 ,至 3 5d形成肉芽组织包裹的油囊结构。结果表明 ,蜂胶疫苗免疫后 ,不但局部组织学影响明显小于油苗注射组 ,而且可趋化大量吞噬细胞到注射局部 ,促进抗原高效递呈 ,且可在注射局部的吞噬细胞胞浆中停留 3 5d以上 ,可能起到了类似“抗原库”的作用 。

Mice were inoculated by gE/gI gene deleted PR inactivated propolis and oil vaccines through leg muscle injection. The muscle tissues on the injection site were collected in the day 2,7,14 and 35 PI. Paraffin tissue section were made by the normal method. The result were:The propolis vaccine was absorbed quickly after injection;There were no abnormal changes compared with the control group; Micoscopically, 2 days PI, light changes in the muscle were observed;7 days PI,there was a kind of macrophage with propolis granules in the cell plasma; More and more such macrophages were observed 14 days PI. Till 35 days PI a mass of such macrophages was formed between the muscle fibers.While the oil vaccine can not be absorbed and stay in the muscle in the injection site as long as the whole experimental period(35 d).Microscopically, fibers rupture,edema or necrosis were very common 2 days PI;there were oil vaccine residues and neutrophil cells between the muscle; Inflammation became even more severe 14 days PI;Granuloma containing oil vaccine was formed at the end of the experiment. The result demonstrated that not only the propolis vaccine has far less effect on the injection tissue than that of oil vaccine, it can also attract a handsome of phagocytes to the injection site, thus raise the efficiency of antigen presenting.Meanwhile,the propolis vaccine was engulfed and stayed long in the cell plasma of phagocytes to exert a role of ′antigen storage′,which could induce the host immune response in a more effective and persistent manner.crosis were very comm