光动力学疗法体外杀灭白血病细胞的初步实验研究

In vitro Leukemia Cells Killed by Photodynamic Therapy: A Preliminary Experimental Investigation

目的研究酞菁锌(ZnPcS2P2)介导的光动力效应体外杀灭K562白血病细胞中光剂量以及光敏剂浓度对白血病细胞存活率的影响。方法用17种不同光剂量(360、324、290、250、200、180、124、90、76、47、29、18、11、6.9、4.2、2.1和0J/cm2)的半导体激光分别辐照与7种不同光敏剂浓度〔0(光照对照组)、0.0625、0.125、0.25、0.5、1.0和2.0μg/ml〕ZnPcS2P2行体外培养的K562白血病细胞。辐照后将细胞置于37℃、5%CO2的培养箱中培养2d,用四唑盐(MTT)比色法测定白血病细胞的存活率。结果单纯药物对K562白血病细胞没有影响,单纯光照在光剂量为360J/cm2时能引起细胞的损伤。与光敏剂共培养的各组白血病细胞均受到激光辐照的不同程度的影响,存活率随着光剂量以及光敏剂浓度的增加而减小。结论光动力学疗法能够有效地杀灭白血病细胞,但是光剂量和光敏剂浓度的选择十分关键。ZnPcS2P2浓度1.0μg/ml、光剂量6.9J/cm2是杀灭K562白血病细胞一组比较理想的搭配。

Objective To investigate the influence of light dose and concentration of photosensitizer on the survival rate of in vitro K562 killed by photodynamic effect with ZnPcS2P2. Methods Seventeen different light doses with seven different concentrations of photosensitizer were used to kill K562 leukemia cells,respectively.The irradiated leukemia cells were incubated in incubator for two days,and the survival rate of leukemia cells was assessed by means of MTT method. Results Medicament alone had no effect on leukemia cells,while laser alone could partly injure leukemia cells under irradiation of high light dose.The survival rate of experimental leukemia cells group decreased with increase in light dose or concentration of photosensitizer. Conclusions The selection of light dose plays an important role on the effect of photodynamic therapy,which could kill K562 leukemia cells effectively when concentration of ZnPcS2P2 and light dose are 1.0 μg/ml and 6.9 J/cm2,respectively.