原核期胚胎的原核模式与胚胎发育及妊娠关系的研究

Relationship between Different Pronuclear Patterns and Potential of Embryo Development and Pregnancy

目的:探讨原核期胚胎的原核模式与胚胎发育及妊娠能力的关系。方法:在IVF/ICSI后16～18 h,根据原核期胚胎的核仁前体数目和分布,将其分为模式0～5;并进行随后的胚胎形态学评估和计算妊娠率。结果:模式0的优良胚胎率(83.13％)高于其它各模式之和(76.11％,P<0.05);单纯移植模式0、含模式0和不含模式0组的妊娠率分别为48.08％、32.14％和21.28％,三组间差异有显著性(P<0.05)。结论:原核期胚胎的原核模式对预测胚胎质量和种植能力有一定参考价值。

Objective: To explore the relationship between the patterns of pronucleus and embryo development and pregnancy potential in the pronuclear stage. Methods:According to the number and distribution of nu-cleolar precursor bodies, the embryos at pronuclear stage were classified into 6 pronuclear patterns from 0 to 5, 16-18 h after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). For each pattern, the subsequent embryonic morphology and the pregnancy rate were analyzed. Results: Embryos of Pattern 0 developed to significantly more embryos with good quality and higher pregnancy potential than the embryos developing from other patterns (83.14% and 76. 11% respectively, P<0. 05). The pregnancy rate was decreased as less embryos of Pattern 0 were transferred . The pregnancy rate of the groups of only Pattern 0, with Pattern 0, and without Pattern 0 were 48. 08%, 32. 14% and 21. 28% respectively (P<0. 05). Conclusion: The pronuclear patterns are of the predictive value of embryo development and pregnancy potential, which can be used as a new tool for the selection of embryos in IVF and ICSI.