摘要
A novel lipid occurred when cyanobacterium Synechocystis sp. PCC 6803 cells were grown in BG-11 medium with glucose applied. This lipid was determined to be a glycolipid, designated glycolipid-x (Glyco-x), by staining with alpha-naphthol and concentrated sulfuric acid. The occurrence of Glyco-x accompanies the disappearance of other lipids, especially DGDG. Glyco-x can also be observed in cells grown in BG-11 medium with the application of other carbon sources: fructose, maltose and lactose. Sodium thiosulfate, an effective scavenger of reactive oxygen intermediates, showed strong capability to inhibit glucose-induced occurrence of Glyco-x. In the presence of 0.3% sodium thiosulfate, Glyco-x could only be detected in cells grown in BG-11 medium with 100 mmol/L glucose applied in late-exponential phase. These results suggest that reactive oxygen species might be involved in the occurrence of Glyco-x in cyanobacterium Synechocystis sp. PCC 6803 cells grown in the presence of glucose.
当蓝细菌Synechocystissp .PCC 6 80 3在添加葡萄糖的BG_11培养基中培养时 ,细胞出现了一种新脂。这种脂经浓硫酸 /α_萘酚染色反应证实为糖脂 ,记为糖脂_x。这一糖脂的出现伴随着其他脂、尤其是双半乳糖甘油二酯含量的下降。此外 ,在添加果糖、麦芽糖、乳糖等其他碳源的培养基中生长的细胞中也检测到这一糖脂。活性氧猝灭剂硫代硫酸钠加入到培养基中能有效地抑制糖脂x的出现。当在BG_11培养基中加入 0 .3%硫代硫酸钠后 ,糖脂x仅能在培养基中添加高浓度 (10 0mmol/L)的葡萄糖且细胞生长处于后指数期时检测到。这些结果表明蓝细菌Synechocystissp .PCC 6 80
基金
中国博士后科学基金
国家重点基础研究发展规划项目 (G19980 10 10 0 )
中国科学院百人计划
中国科学院植物研究所光合作用基础研究实验室创新项目。~~
