化学比色法测定发酵液中L-缬氨酸的研究

Study on Chemical Colorimetry Determining L-valine in Fermented Broth

应用“化学比色法”对发酵液中L -缬氨酸进行了定量分析研究 ,确立了L -缬氨酸定量测定条件和计算方法。以高速氨基酸分析仪测定结果为参比 ,用化学比色法测定结果的准确度比纸层析 -色斑洗脱比色法高 ,且应用化学比色法测定L-缬氨酸的含量较为方便。

L-valine concentration in fermented broth was quantitatively determined with methods of chemical colorimetry. The quantitative determination conditions and computing method were established. Compared with high-speed amino acid analyzer, the determination value of L-valine concentration with chemical colorimetry was more accurate and convenient than with paper chromatography-mottle elution colorimetry.