自身免疫性疾病患者血清可溶性CD4、CD8的测定及临床意义

The determination of serum soluble CD4 and CD8 antigen in patients with autoimmune diseases

目的 探讨血清可溶性CD4、CD8抗原 (sCD4、sCD8)在自身免疫性疾病中测定的临床意义。 方法采用ELISA法测定78例Graves病 (GD)、系统性红斑狼疮 (SLE)和类风湿关节炎(RA)患者和38名健康者 (对照组 )的血清sCD4、sCD8水平 ,并对28例GD患者血清sCD4、sCD8水平作治疗前后的动态观察。 结果 3组患者治疗前血清sCD4、sCD8水平均高于对照组[sCD4对照组 (4.16±2.7)u/ml,GD和SLE患者为 (8.05±4.19)u/ml和(7.93±4.12)u/ml,与对照组比P<0.01 ,RA患者 (6.27±3.24)u/ml ,与对照组比P<0.05 ;sCD8对照组 (4.27±2.17)u/ml,GD、SLE、RA患者分别为 (11.37±4.34)u/ml、(15.38±5.65)u/ml、(12.25±4.48)u/ml,与对照组比均P<0.01)。经丙基硫氧嘧啶治疗后 ,GD患者sCD8下降。血清sCD4、sCD8与TT3、TT4、TSH、ANA、C3、ESR、RF无相关性。 结论 GD、SLE、RA患者的血清sCD4、sCD8水平升高 ,可能是独立的免疫学监测指标。

Objective To investigate the clinical value of serum soluble CD4and CD8antigen(sCD4,sCD8)in patients with autoimmune diseases.Methods The levels of serum sCD4and sCD8were detected by ELISA in78patients with Graves disease(GD),Systemic Lupus Erythematosus(SLE)and Rheumatoid arthritis(RA);serial measurements were carried out in28GD patients before and after treatment with propythiouracil(PTU).Results The levels of serum sCD4and sCD8were elevated significantly in GD,SLE and RA paˉtients.After treated with PTU,serum sCD8level returned to normal and euthyroidism was established in28GD patients.There was no significant correlation of sCD4and sCD8with T3,T4,TSH,ANA,C3,ESR,RF.Conclusion The serum sCD4and sCD8levels in GD,SLE and RA patients were elevated significantly,which indicates that these two soluble antigens might be used as independent markers for monitoring immunoˉlogical status of autoimmune diseases.