纤维素-三(3,5-二甲基苯基氨基甲酸酯)对烯唑醇对映体的直接拆分

Direct Resolution of Diniconazole Optical Enantiomers on a Cellulose-tris(3,5-dimethylphenylcarbamate) Chiral Stationary Phase

制备了纤维素-三(3,5-二甲基苯基氨基甲酸酯)手性固定相(CDMPC),以正己烷为流动相,添加异丙醇为改性剂,在高效液相色谱上实现了对烯唑醇光学异构体的直接拆分,探讨了改性剂的含量及色谱柱长对拆分效果的影响,从而优化了拆分条件。试验结果显示,流动相中异丙醇含量的减少有利于对映体的拆分,但保留增强;长色谱柱也有利于对映体的分离。使用两色谱柱串连,当流动相中正己烷与异丙醇的比例为95∶5时可达到最佳分离效果,分离因子为1. 17。

Cellulose-tris(3,5-dimethylphenylcarbamate) (CDMPC) chiral stationary phase (CSP) was synthesized. The reso-lution of tebuconazole racemates on the CSP was realized on high-performance liquid chromatography(HPLC) using an n-hexane: isopropanol mobile phase. The effect of isopropanol concentration in the mobile phase and the effect of column lengthwere investigated, and chromatographic conditions were optimized. The results showed that low percentage of isopropanol (5%)and a long column length provided best separation.