近无柄金丝桃中的口山酮类成分

Xanthone constituents of Hypericum subsessile

在细胞毒性试验结果指导下,同步对近无柄金丝桃(HypericumsubsessileN.Robson)全株的有效部位进行化学成分分离纯化,得6个口山酮类化合物,通过理化数据测定及波谱数据分析,鉴定了结构,分别是:2,3-二甲氧基口山酮(1),1,3-二羟基-5,6-二甲氧基口山酮(2),ToxyloxanthoneB(3),Kielcorin(4),CandensinD(Hypericorin)(5)和Subalatin(6).化合物(1)～(6)均为首次从该植物中分离得到;同时,细胞毒试验发现,近无柄金丝桃的氯仿及乙酸乙酯提取物有一定细胞毒活性,其IC50分别为8.8,14.6μg/mL.

Cytotoxicity screening of the extracts of Hypericum subsessile N.Robson as carried out by L_(1210) cells.Isolation of chemical constituents as carried out from the effective parts of H.subsessile by column chromatography. The chemical structures were identified by physico-chemical properties and spectral data.Six xanthone constituents have been isolated and elucidated as 2,3- dimethoxyxanthone (1),1,3- dihydroxy-5,6- dimethoxyxanthone (2),toxyloxanthone B (3),kielcorin (4),candensin D(Hypericorin) (5) and subalatin(6). Compounds 1～6 were obtained from H.subsessile for the first time.In addition,CHCl_3 and EtOAc fractions possess cytotoxic activities and the IC_(50) are 8.8 and 14.6?μg/mL for L_(1210) cells,respectively.