紫外吸光光度法测定厚朴酚及和厚朴酚的解离常数

Determination of the dissociation constants of magnolol and honokiol by UV spectrophotometry

根据光度法测定酸解离常数的原理,采用紫外分光光度法测定了厚朴酚与和厚朴酚的解离常数(pKa),并用计算机和统计方法作数据处理.实验测得厚朴酚的解离常数pKa1为7.10±0.11,pKa2为10.58±0.36,和厚朴酚的pKa1为9.64±0.03,pKa2为10.71±0.21.方法简便,结果可靠准确.

According to the principal of determination of acidic dissociation constant by spectrophotometry,the UV spectrophotometric method was used to determine the dissociation constants of magnolol and honokiol(pKa).The data were processed with statistics method via computer The result shows that pKa_1 and pKa_2 of magnolol are 7.10±0.11 and 10.58±0.36,and pKa_1 and pKa_2 of honokiol are 9.64±0.30 and (10.71)±0.21,respectively.This method is simple and result is reliable and accurate.