以苦丁茶为原料提取熊果酸工艺的研究

Study on Extracting Technique of Ursolic Acid from Ilex Kudingcha

采用正交设计L16(45) ,用高效液相色谱法对不同提取工艺条件下苦丁茶提取液中的熊果酸的含量进行测定 ,并计算出各提取工艺条件下熊果酸的提取率。筛选出从苦丁茶中提取熊果酸的最佳工艺条件。结果显示苦丁茶中熊果酸的最佳提取工艺条件为A4B4C3 D2 ,即 95 %乙醇为溶剂 ,料液比 1∶12 ,水浴回流 3h ,提取 3次 ,提取率达 1.76 9%。

By determining the content of ursolic acid in extracting solution of different extracting technique with HPLC by the orthogonal designed methods (L 16 (4 5) and calculating the extraction rate of ursolic acid, the optimized extracting technique of ursolic acid from Ilex kudingcha was determined. Results showed the optimum extracting technique was A 4B 4C 3D 2, the extraction rate reached 1.769 % when the extraction was conducted three times using 95 % ethanol as solvent for three hours each time and the mass ratio of ethanol to raw material was 1∶12.