尼膜同对戊四氮致痫大鼠海马神经细胞凋亡和p^(53)蛋白表达的影响

Effects of Nimotopine on neuronal apoptosis and p^(53) protein expression in hippocampal area of the rats with pentylenetetrazol-induced epilepsy

目的 :探讨尼膜同对癫痫发作大鼠海马神经细胞凋亡与 p53蛋白表达的影响。方法 :采用戊四氮致痫大鼠模型 ,以原位末端标记 ( TUNEL)法检测凋亡细胞 ;免疫组化法检测 p53蛋白。观察大鼠癫痫发作后 48h其海马 CA1区神经细胞凋亡和 p53蛋白表达变化及尼膜同对它们的影响。结果 :戊四氮致痫后 ,大鼠海马 CA1区凋亡细胞和 p53蛋白表达均明显增加 ,p53蛋白表达与细胞凋亡呈正相关 ( r=0 .846,P<0 .0 0 1 ) ;尼膜同干预后 ,凋亡细胞数及 p53蛋白表达均显著性降低 ( P<0 .0 0 1 )。结论 :尼膜同对癫痫所致神经细胞凋亡有抑制作用 ,其抑制凋亡的分子机制可能是通过抑制

Objective:To study the effects of Nimotopine (NM) on the neuronal apoptosis and p 53 protein expression in the hippocampal area of the rats with pentylenetetrazol(PTZ)-induced epilepsy. Methods:The model of PTZ- induced epilepsy was used,TUNEL technique and immunohistochemistry method were used to measure the neuronal apoptosis and expression of p 53 protein.After the epilepsy stopped for 48h , effects of NM on the neuronal apoptosis and p 53 protein expression in the hippocampus CA 1 region of the rat were observed. Results:After PTZ- induced epilepsy, apoptotic neurons and p 53 protein expression increased significantly.Correlation analysis showed that neuronal apoptosis had positive correlation with p 53 protein expression(r=0.846,P<0.001). Apoptotic neurons and p 53 protein expression in NM groups were markedly less than those in epilepsy group(P<0.001). Conclusion: NM can inhibit the hippocampal neuronal apoptosis and p 53 protein expression induced by acute seizures, molecular mechanisms of which may be attributed to inhibit p 53 protein expression.