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中华蜜蜂与意大利蜜蜂中肠消化酶活性差异性探讨 被引量:4

Inquisition of the midgut digestive enzyme activity difference between A.c.cerana and A.m.ligustica
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摘要 本试验对中华蜜蜂 (A .c .ceranaFab )和意大利蜜蜂 (A .m .ligusticaSpinola)的成年工蜂、幼虫中肠内蛋白酶、淀粉酶、蔗糖酶的活性进行了比较。试验结果显示 :中华蜜蜂、意大利蜜蜂幼虫之间三种酶活性差异均不显著 ;而中华蜜蜂、意大利蜜蜂的成蜂间蛋白酶活性差异显著 (P <0 0 5 ) ,意大利蜜蜂高于中华蜜蜂 ;淀粉酶和蔗糖酶的活性分别为差异显著 (P <0 0 5 )、差异极显著 (P <0 0 1 ) ,中华蜜蜂高于意大利蜜蜂 ;同一蜂种内 ,成年蜂中肠液内的三种酶活性 ,均高于大、小幼虫 ,且差异极显著 (P <0 0 1 )。 The activities of protease, amylase and sucrase in the midguts of adult worker bees and larvae were compared with Apis cerana cerana Fabricius and Apis mellifera ligustica Spionla. The results indicated that:three enzymes activities were no siginificant difference between A.c.cerana larvae and A.m.ligustica larvae;the protease activity in the adults midguts of A.m.ligustica was higher than that of A.c.cerana , the difference was marked (p<0.05); the amylase and sucrase activities in adults midguts of A.c.cerana were higher than that of A.m.ligustica , the differences were respectively marked(p<0.05), extremely marked(p<0.01); for one species, the activities of three digestive enzymes in the midguts of adult worker bees were stronger than those of larvae, and the differences were extremely marked(P<0.01).
出处 《中国养蜂》 2004年第1期4-5,共2页 Apiculture of China
关键词 中华蜜蜂 意大利蜜蜂 肠消化酶 活性 成年工蜂 幼虫 蛋白酶 淀粉酶 蔗糖酶 Apis cerana cerana Fabricius Apis mellifera ligustica Spinola Protease Amylase Sucrase Activity
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参考文献3

  • 1缪晓青,李江红,吴珍红,张其康,孙亮先,陈刚.蜜蜂消化道酶谱带分布及其糖转化酶活性[J].福建农业大学学报,2000,29(1):95-98. 被引量:3
  • 2Delage-Darchen B,Barchen R. Digestive enzymes of the salivary glands and the midgut of a social Mexican bee, M.elipona beecheii, Annals des Sciences Naturelles Zoologie, 1982,4(2) :91--96.
  • 3Smirle M J, Winston M L. Detoxifying enzyme activity in worker honeybees: an adaptation for foraging in contaminated ecosystems.Canadian Journal of Zoology, 1988,66(9) : 1938 - 1942.

二级参考文献1

  • 1张龙翔,生化实验方法和技术,1982年,94页

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