兔肺小动脉内羊水栓塞成分的时序变化及其意义

Sequential changes of the component of the amniotic embolism in the pulmonary a rteriole of rabbit and its significance

目的 :观察羊水成分在肺小动脉内时序演变过程 ,为羊水栓塞诊断提供依据。方法 :建立兔羊水栓塞症模型 ,用HE ,阿尔辛兰 荧光桃红改良染色法及TEM法 ,观察羊水中细胞进入兔肺后的时序变化及病变过程。结果 :角化上皮等进入兔肺血管后经 15d即分解消失 ,但其引起的多发性结节性小动脉炎经 2 2d才可见到 ,TEM可见到角化上皮的凋亡过程。结论 :羊水中角化上皮细胞在肺小动脉内的时序改变及继发血管改变在病理诊断医疗事故鉴定中具有重要意义。

Objective: To observe the sequential changes of the components of the amniotic embolism in the pulmonary arteriole of rabbit and to provide the diagnostic evidence for th e amniotic embolism.Methods:A model with amniotic embolism in rabbits was made,then the sequential changes and the pathological process of the amniotic cell in vaded into the lungs of the rabbit were observed with H￣E and the modified Alci n blue￣fluorescent pink staining under TEM. Results:The keratinized epithelia dissolved after it invaded th e lung of the rabbit and eliminated in 15 days,but the multiple nodular arteriol itis caused by keratinized epithelia could be seen even after 22 days.Conclusion:The sequential changes of the amniotic embolism in t he pulmonary arterioles of rabbit and the secondary vascular changes have import ant significance in identification for medical negligence.