革兰阴性杆菌中超广谱β-内酰胺酶和AmpC酶的检测与分析

Detection and analysis of extended-spectrum β-lactamase and AmpC enzyme in Gram-negative bacteria

目的 :了解革兰阴性杆菌中超广谱 β 内酰胺酶 (ESBLs)、AmpC酶的产生情况 ,为临床用药提供参考依据。方法 :用纸片扩散确证试验检测 3 4 5株革兰阴性杆菌的ESBLs产生情况 ,同时用酶提取物三维试验检测其中 194株菌的AmpC酶产生情况。结果 :革兰阴性杆菌中 ,ESBLs阳性率为 3 0 .4% ,主要产生菌为阴沟肠杆菌 68.2 % ,大肠埃希菌 45.9% ,肺炎克雷伯菌 3 1.0 % ;AmpC酶阳性率为 11.9% ,主要产生菌为阴沟肠杆菌 2 9.5% ,鲍曼不动杆菌 18.2 %。结论 :在革兰阴性杆菌中ESBLs、AmpC酶产生情况已相当严重和普遍 。

Objective: To investigate the production and distribution of extended-spectrum β-lactama se,AmpC enzyme in Gram-negative bacteria, and to provide references for the clinical application of drugs. Methods:ESBLs were detected against 345 strains by disc diffusi on confirmatory test,AmpC were detected against 194 strains by three dimensional test.Results:Among 345 strains,producing ESBLs bacteria accounted f o r 30.4%,of them Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae we re 68.2%,45.9% and 30.0% respectively;Among 194 strains producing AmpC enzyme ba cteria accounted for 11.9%(23/194),of them Enterobacter cloacae,Acinetobacter ba umannii were 29.5% and 18.2% respectively. Conclusion:The production situation of ESBLs and AmpC enzyme in the gram-negative ba cteria are considerably severe and prevalence and should be detected and treated carefully.