期刊文献+

锌指蛋白ZNF191(243-368)G321A;N324L;G321A/N324L突变体的制备和表征 被引量:2

Preparation and Characterization of Zinc Finger Protein ZNF191(243-368)'s Mutants
原文传递
导出
摘要 通过分子生物学的蛋白质工程方法对锌指蛋白ZNF191(243 368)的第三锌指区Gly321和Asn324进行了定点突变,在大肠杆菌BL21中表达,通过DEAE52,CM23,肝素柱CL 6B纯化,最终获得了ZNF191(243 368)G321A;N324L;G321A/N324L等3个突变体蛋白.运用凝胶电泳、UV光谱、EDTA滴定、金属离子(Co2+,Ni2+)取代等方法对突变体蛋白进行了初步表征.这为今后进一步研究该蛋白的结构和功能提供了必要的物质基础. Several amino acid residues on the third finger of the zinc finger protein ZNF191(243-368) are mutated by four primer PCR method. These mutants are expressed in pTSA-18/E.coli BL21(DE3) system and purified through DEAE52, CM23, Heparin Sepharose CL-6B chromatographic colomns. The mutant proteins are characterized and studied by electrophoresis, Ultra-Visible spectrum, EDTA titration and Co^(2+), Ni^(2+) substitution. The results suggested that the mutations of the chosen amino acid residues do not affect the coordination between Zn^(2+) and cysteines of the protein.
出处 《复旦学报(自然科学版)》 CAS CSCD 北大核心 2003年第6期846-850,共5页 Journal of Fudan University:Natural Science
基金 国家自然科学基金资助项目(29671008)
  • 相关文献

参考文献2

二级参考文献3

共引文献11

同被引文献17

  • 1余晓丹,沈晓明,颜崇淮.锌指蛋白结构和功能研究进展[J].国外医学(卫生学分册),2004,31(3):171-175. 被引量:16
  • 2胡华军,陈勇,洪艳.甲型肝炎病毒受体HAVcr-1及其与过敏性疾病的关系[J].国外医学(流行病学.传染病学分册),2005,32(3):168-170. 被引量:2
  • 3龙贤辉,徐勤枝,贺性鹏,安静,隋建丽,白贝,周平坤.2Gy和10Gyγ射线照射正常人淋巴母细胞基因表达转录谱的比较[J].中华放射医学与防护杂志,2006,26(2):110-113. 被引量:7
  • 4Amundson S A, Billerner M, Melher P,et al. Induction of gene express as a monitor of exposure to ionizing radiation. Radial. Res., 2001. 156(5):657.
  • 5Christiansen H, Batusic D, Saile B, et al. Identification of genes responsive to gamma radiation in rat hepatocytes and rat liver by cDNA array gene expression analysis. Radiat. Res., 2006. 165(3):318.
  • 6Sharma R, Lemasters J, Herman B. Lipid order in hepatocyte plasma membrane blebs during ATP depletion measured by digitized fluorescence polarization microscopy. Int. J. Radiat. Biol., 1993. 63 (2): 233.
  • 7Raft M C. Social controls on cell survival and cell death. Nature, 1992. 356(2) :397.
  • 8Schena M, Shalon D, Davis R W,et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science, 1995. 270 (5235) : 467
  • 9Schena M, Shalon D, Heller R, et al. Parallel human genome analysis: Microarray based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA, 1996.93 (20) : 10614
  • 10Sharma R, Lemasters J, Herman B. Lipid order in hepatocyte plasma membrane blebs during ATP depletion measured by digitized fluorescence polariza- tion microscopy. Int. J. Radiat. Biol. , 1993.63 ( 2 ) : 233

引证文献2

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部