家蝇Prohibitin基因的生物信息学分析

Bioinformatics analysis of Prohibitin gene of Musca domestica(Housefly)

为对家蝇Prohibitin蛋白序列进行生物信息学分析,从而为该基因功能研究奠定基础。利用在线分析程序和相关工具软件分析Prohibitin蛋白的理化性质、结构域、并预测其空间结构和功能。结果表明家蝇Prohibitin蛋白由277个氨基酸组成,分子量为30.54 k Da,理论等电点为5.26,为稳定蛋白,有跨膜区,但不含信号肽,该蛋白属于PHB保守结构域家族,亚细胞定位于细胞质,二级结构以α-螺旋为主。蛋白同源性比对结果显示,昆虫中的Prohibitin蛋白具有较高的同源性。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。

To perform the bioinformatics analysis of Prohibitin gene of Musca domestica( Housefly),and aims at providing the basis for its' function research. Internet online procedures and the related software are exploited to analyze the physical and chemical properties of Prohibitin protein and the domains of the protein,and predict the structure and functions of the protein. The Prohibitin protein sequence is composed of 277 amino acids with30.54 k Da of the molecular weight. Theoretical isoelectric point is 5.26. The protein is stable and had structures of transmembrane and helical coils. The protein belongs to the family of PHB,and is located in cytoplasm and dominated by alpha helix in second structure. The phylogenetic tree reveals that the Prohibitin protein in insect has the highest homology. This work provides a systemic sequence analysis of Prohibitin protein of Musca domestica( Housefly). It will provide the useful reference for further investigation of its structure and function.