Raf和Spry蛋白在宫颈鳞状细胞癌组织中的表达及临床意义

Significance of the expression of Raf and Spry protein in cervical squamous cell carcinoma tissues

目的探讨Raf和Spry蛋白在宫颈鳞状细胞癌组织中的表达及临床意义。方法选择2013年1月至2015年12月河南科技大学第一附属医院病理科保存的宫颈组织石蜡切块133例,其中宫颈鳞状细胞癌50例,高度上皮内病变32例,低度上皮内病变22例,正常宫颈组织29例。采用免疫组织化学法检测Raf、Spry蛋白在不同宫颈组织中的表达,分析Raf、Spry蛋白表达与宫颈鳞状细胞癌患者临床病理特征的关系及Raf与Spry蛋白的相关性。结果随着宫颈组织病变的加重,Raf蛋白阳性表达率呈逐渐升高趋势,Spry蛋白阳性表达率呈逐渐降低趋势;Raf、Spry蛋白在不同宫颈组织中的表达两两比较差异均有统计学意义(P<0.05)。Raf蛋白表达与宫颈鳞状细胞癌的临床分期、淋巴结转移有关(P<0.05),与患者年龄、病理分级无关(P>0.05);Spry蛋白表达与宫颈鳞状细胞癌的病理分级有关(P<0.05),与患者年龄、临床分期及淋巴结转移无关(P>0.05);Raf与Spry蛋白表达呈负相关(r=-0.460,P<0.05)。结论 Raf和Spry蛋白与宫颈鳞状细胞癌的发生、发展有关,二者在宫颈鳞状细胞癌组织中的表达呈负相关。

Objective To investigate the expression and significance of Raf and Spry protein in cervical squamous cell carcinoma.Methods One hundred and thirty-three cervical tissues paraffin sections which were preserved in the Department of Pathology,the First Affiliated Hospital of Henan University of Science and Technology from January 2013 to December 2015 were selected.Among them,there were 50 cases of cervical squamous cell carcinoma,32 cases of highly intraepithelial lesions,22 cases of low-grade intraepithelial lesions and 29 cases of normal cervical tissues.The expression of Raf and Spry protein in different cervical tissues were detected by immunohistochemistry.The relation between the expression of Raf,Spry protein and the clinicopathological characteristics of cervical squamous cell carcinoma patients was analyzed.The correlation of the expression of Raf and Spry protein in cervical squamous cell carcinoma was also analyzed.Results With the aggravation of cervical lesions,the positive expression of Raf protein increased gradually,and the expression of Spry protein decreased gradually;there was significant difference in Raf,Spry protein expression among any two cervical tissues(P<0.05).The expression of Raf protein was associated with clinical stage and lymphatic metastasis of cervical squamous cell carcinoma patients(P<0.05),but it was not associated with the age and pathological grade of patients(P>0.05);the expression of Spry was associated with pathological grade of cervical squamous cell carcinoma patients(P<0.05),but it was not associated with the age,clinical stage and lymphatic metastasis of patients(P>0.05).The expression of Raf protein was negatively correlated with Spry protein(r=-0.460,P<0.05).Conclusion The expression of Raf and Spry protein are involved in the development of cervical squamous cell carcinoma.The expression of Raf and Spry protein in cervical squamous cell carcinoma tissues is negatively correlated.