星点设计-效应面法优化酶法提取黄连中盐酸小檗碱工艺

Optimization of Enzymatic Extraction Process in Berberine Hydrochloride from Coptidis Rhizoma by Central Composite Design and Response Surface Method

目的研究黄连中盐酸小檗碱的酶法辅助超声提取工艺,并优化酶解工艺参数。方法采用果胶酶酶解后超声促提方法提取黄连中的盐酸小檗碱,在单因素试验的基础上,选取酶用量、酶解温度和酶解p H值为影响因素,通过星点设计-效应面法优化酶解工艺参数,并利用多元线性回归和二项式回归建立预测模型。结果最佳酶解工艺为酶用量1.33%、酶解温度45.8℃、酶解p H值2.68,在该优化条件下,盐酸小檗碱提取率为96.3%,与模型预测值(97.0%)相差0.7%。结论采用酶法辅助超声提取可充分提取出黄连中的盐酸小檗碱,且利用二项式回归所建立的模型具有较好的预测性。

Objective To study the enzymatic assisted ultrasonic extraction process of berberine hydrochloride from Coptidis Rhizoma;To optimize enzymatic hydrolysis parameters.Methods Berberine hydrochloride was extracted from Coptidis Rhizoma by pectinase enzymatic hydrolysis and ultrasonic extraction.Base on single factor experiments,three factors of enzyme dosage,enzymolysis temperature,and pH value were chosen to optimize the pectinase extraction conditions by central composite design and response surface method.The prediction model was established by multiple linear regression and binomial regression.Results The optimum enzymatic hydrolysis process was as follows:the enzyme dosage 1.33%,the enzymolysis temperature 45.8℃,and the pH value 2.68.Under these conditions,the extraction rate of berberine hydrochloride was 96.3%,which was different from the model prediction(97.0%)by 0.7%.Conclusion The method of enzymatic assisted ultrasonic extraction can be used to extract berberine hydrochloride from Coptidis Rhizoma.Meanwhile,the established binomial regression model has better predictability.