IGFBP3在急性髓系白血病中的表达及临床意义

Expression of Insulin-like Growth Factor Binding Protein 3 in Acute Myeloid Leukemia and Its Clinical Significance

目的:探讨胰岛素样生长因子结合蛋白3(insulin-like growth factor binding protein3,IGFBP3)在急性髓系白血病的表达及临床意义。方法:以GAPDH为内参,应用SYBR-GreenΙ实时荧光定量PCR法检测IGFBP3基因在43例AML初治(de novo AML)患者、36例完全缓解(CR)患者、28例对照者的骨髓单个核细胞中的表达情况,比较三组基因表达的差异。结果:初治组的IGFBP3基因表达水平均低于CR组和对照组(P<0.05),CR组与对照组之间无统计学差异(P>0.05)。结论:IGFBP3可能作为一种抑癌基因在急性髓系白血病的发病中起到一定的作用,完全缓解后表达水平恢复,其可以作为一种评估临床疗效的指标。

Objective:To explore the expression of insulin-like growth factor binding protein 3(IGFBP3) in acute myeloid leukemia and its clinical significance.Methods:Using GAPDH as internal reference,IGFBP3 gene expression was detected in the bone marrowmononuclear cells of 43 de novo AML patients,36 patients with complete remission(CR) and 28 cases of controls by using SYBR-Green I real-time quantitative PCR,the differences of gene expression between the three groups were compared.Results:IGFBP3 gene expression level in the de novo group were lower than that in CR group and control group(P < 0.05),but there was no statistically significant difference of IGFBP3 expression level in CR group and control group(P > 0.05).Conclusions:The IGFBP3 as a tumor suppressor gene may play a role in the pathogenesis of acute myeloid leukemia,its expression level is recovered after complete remission,therefore,IGFBP3 can be used as an indicator of evaluating clinical curative effect.