三氧化二砷对慢性髓系白血病细胞Hedgehog通路的影响

Effect of As_2O_3 on Hedgehog Pathway in Chronic Myeloid Leukemia Cells

目的:探讨三氧化二砷对慢性髓系白血病(CML)细胞Hedgehog信号通路的影响。方法:采用MTT法及流式细胞术测定细胞的凋亡,Western blot检测Hedgehog通路中PTCH及SMO蛋白表达,real-time PCR检测PTCH及SMO基因mRNA的表达。结果:三氧化二砷可诱导K562细胞凋亡,最佳浓度为2μmol/L,最佳作用时间为24 h。在该浓度及时间点作用下Hedgehog通路中PTCH及SMO蛋白和mRNA表达下调。结论:三氧化二砷可降低Hedgehog通路中PTCH及SMO基因表达。

Objective:To explore the effect of As_2O_3 on Hedgehog pathway in chronic myeloid leukemia(CML)cells.Methods:The apoptosis of K562 cells was detected by MTT method and flowcytometry;the expressions of PTCH and SMO protein and mRNA in Hedgehog pathway were determined by Western blot and real-time PCR,respectively.Retults:The As_2O_3 could induce the apoptosis of K562 cells with optimal concentration 2 μmol/L and optimal time 24 hours.The expressions of PTCH and SMO protein and mRNA in Hedgehog pathway of K562 cells treated with As_2O_3 at optimal concentration and optimal time were down-regulated.Conclusion:The As_2O_3 can downregulate the expression of PTCH and SMO in Hedgehog pathway.