姜黄素对CD34^+人急性髓系白血病细胞增殖抑制作用及机制探讨

Inhibitory Effect of Curcumin on Proliferation of CD34^+ Acute Myeloid Leukemia Cells and Its Mechanism

目的:探讨姜黄素对CD34+人急性髓系白血病细胞株KG1a和Kasumi-1增殖抑制作用及其机制。方法:以不同浓度姜黄素(0、20、40、80μmol/L)处理KG1a和Kasumi-1细胞48 h,采用台盼蓝染色计数法检测细胞活率,Western blot法测定姜黄素对细胞NF-κB P65核蛋白表达影响,免疫荧光检测姜黄素对细胞NF-κB P65核蛋白移位影响。结果:姜黄素可显著抑制KG1a和Kasumi-1细胞生长,随着姜黄素浓度的增加,细胞数量逐渐下降。姜黄素可下调KG1a和Kasumi-1细胞P65核蛋白表达,抑制NF-κB P65核移位,使NF-κB处于失活状态。结论:姜黄素可抑制KG1a和Kasumi-1细胞增殖,其机制可能与姜黄素抑制NF-κB P65核蛋白表达有关。

Objective:To explore the inhibitory effect of curcumin on proliferation of CD34^+ acute myeloid leukemia cells and its mechamism.Methods:KGla and Kasumi-1cell lines were treated with curcumin of different concentrations(0,40,60,80 μmol/L).The effect of curcumin on cell viability and proliferation was detected by trypan blue staining and cell count.The effect of curcumin on distribution of NF-kB P65 subunit was analyzed by immunofluorescence and Western blot.Results:The curcumin inhibited proliferation of KGla and Kasumi-l cells in a dose-dependent manner.Western blotting showed that curcumin led to significant down-regulation of NF-kB P65 nuclear protein expression,Immunofluorescence assay showed that treatment with 40 μmol/L of curcumin for 48 h suppressed the nuclear translocation of NF-kB p65 in KGla and Kasumi-l cells.Conclusion:The curcumin suppresses cell growth of KGla and Kasumi-1 cells,its mechanism may be related to inhibitory effect of curcumin on NF-kB p65 nucleus protein.