骨髓增生异常/骨髓增殖性肿瘤-不能分类的差异表达的信号通路蛋白分析

Analysis of Differentially Expressed Signaling Pathway Proteins in Myelodysplastic/Myeloproliferative Neoplasms——Unclassifiable

目的:对骨髓增生异常/骨髓增殖性肿瘤-不能分类(MDS/MPN-U)的信号通路蛋白进行筛选,探讨差异表达的蛋白在MDS/MPN-U发病机制中可能涉及的作用。方法:采用蛋白通路芯片技术(protein pathway array,PPA)比较10例M DS/M PN-U患者和10例正常对照中性粒细胞信号通路蛋白表达差异,并通过蛋白质组学数据应用平台(IPA)分析差异蛋白参与的信号传导通路及建立信号传导网络。结果:与正常对照组比较,MDS/MPN-U组中共有25种蛋白存在差异表达,其中15种蛋白表达上调,10种蛋白表达下调。这些蛋白主要参与影响10个信号传导通路,这些通路与细胞增殖和免疫相关,并且相互作用,形成复杂信号传导网络。结论:采用PPA技术筛选M DS/M PN-U信号通路蛋白差异表达谱,有利于揭示M DS/M PN-U发病机制及寻找新的治疗靶点。

Objective:To screen signaling pathway proteins in myelodysplastic/myeloproliferative neoplasms unclassifiable(MDS/MPN-U),and to explore the possible role of the differentially expressed signaling pathway proteins in pathogenesis of MDS/MPN-U.Methods:Protein Pathway Array(PPA) was applied to analyze the differential expression levels of signaling pathway proteins in 10 patients with MDS/MPN-U and normal controls,and furthermore to identify the signaling pathways and network in which these proteins were analyzed by Ingenuity pathway analysis program.Results:The expressions of 25 signaling proteins in MDS/MPN-U were significantly different,compared with the control group.Among them 15 proteins were upregulated in MDS/MPN-U patients,while 10 proteins were downregulated.These dysregulated proteins were involved in 10 major signaling pathways related with cell proliferation and immunity.The complicated interactive network was established by these proteins and pathways.Conclusion:The differentially expressed signaling proteins screened from the MDS/MPN-U patients by PPA might be helpful to reveal the pathogenesis of MDS/MPN-U and to discover the therapeutic targets.