白头翁皂苷A对K562细胞红系诱导分化作用的研究

Differentiation of K562 Cells Induced by Pulsatilla Saponin A into Erythroid Lineage

目的:探讨白头翁皂苷A对K562细胞向红系诱导分化的作用。方法:应用不同浓度的白头翁皂苷A作用于K562细胞后,采用联苯胺染色阳性细胞计数、细胞内血红蛋白测定及流式细胞术检测细胞膜表面CD71及GPA分化抗原表达等方法评价白头翁皂苷A对K562细胞的诱导分化作用。结果:不同浓度的白头翁皂苷A均可提高K562细胞内血红蛋白的含量;上调K562细胞膜表面CD71及GPA分化抗原的表达。其中以4μg/ml的白头翁皂苷A的作用最为显著。结论:白头翁皂苷A可诱导K562细胞向红系分化。

Objective:To explore the differentiation-inducing potentiality of Pulsatilla saponin A on K562 cells.Methods:Pulsatilla saponin A of different concentrations was used to treat K562 cells;the benzidine staining and the hemoglobinometry were applied to measure the change of hemoglobin content;the flow cytometry(FCM) was used to detect the expression of CD71 and GPA on K562 cells.Results:K562 cells treated with 4 μg/ml pulsatilla saponin A differentiated into the erythroid lineage.With the treatment of pulsatilla saponin A,the hemoglobin content in K562 cells increased significantly;CD71 and GPA expression on the K562 cell surface were up-regulated.Conclusion:Pulsatilla saponin A can induce K562 cells to differentiate into erythroid lineage.