树突状细胞促进外周血CRTH2(CD4^+ CD294^+ Th2)细胞增殖和辅助B细胞分泌免疫球蛋白

Dendritic Cells Promote the Proliferation of Peripheral Blood CRTH2 Cells(CD4^+ CD294^+ Th2) and Help B Cells to Secrete Immunoglobulin

目的:研究树突状细胞对CRTH2(CD4^+CD294^+Th2)细胞促增殖作用及CRTH2细胞对B细胞功能的影响,为扩增及分选Th2细胞提供新方法。方法:树突状细胞负载卡介苗抗原后行混合淋巴细胞反应,应用流式细胞仪分选DC及CRTH2细胞,然后将分选的CRTH2细胞与B细胞共培养,检测Ig G及Ig A的分泌。结果:树突状细胞负载卡介苗抗原能够促进CRTH2细胞亚群比例升高,其绝对数也明显升高,与B细胞共培养后上清中Ig G、Ig A和Ig E水平升高,差异有统计学意义(P<0.05)。结论:树突状细胞负载抗原能够促进CRTH2细胞增殖,CRTH2细胞可辅助B细胞分泌免疫Ig G、Ig A和Ig E。

Objective:To investigate the promotive effect of dendritic cells(DCs) on proliferation of CRTH2(CD4^+CD294^+ Th2) cells and the influence of CRTH2 cells on secretion of immunoglobulin from B cells so as to provide a new approach for amplification and sorting of Th2 cells.Methods:DCs were induced from peripheral blood mononuclear cells,then the loaded-BCGV-Ag-DCs were cocultured with T cells,and the mixed lymphocyte reaction(MLR) was performed by CCK8 method.The phenotypes of DCs and CRTH2 cells were detected by flow cytometry.CRTH2 cells sorted by MACS were co-cultured with B cells for 5 days to detect the secretion of immunoglobulin.Results:The subsets and absolute number CRTH2 cells were significantly increased by loaded-BCGV-Ag-DCs.The levels of IgG,IgA and IgE were higher increased in supernatant of CRTH2 and B cell co-culture system than that in control group or that in transwell group(P <0.05).Conclusion:The proliferation of CRTH2 cells can be greatly promoted by loaded-BCGV-Ag-DCs,and the CRTH2 cells can help B cells to secrete IgG,IgA and IgE.