黄芩苷对抑郁症模型小鼠海马钙结合蛋白D28K表达的影响

Effects of baicalin on expression of calbindin-D28K in hippocampus of depression model mice

目的观察黄芩苷对抑郁症模型小鼠的行为学变化及海马细胞中钙结合蛋白D28K (calbindin-D28K)表达的影响。方法选用健康成年ICR小鼠60只,随机分为空白对照组,模型组,阳性对照(氟西汀5.2 mg·kg^(-1))组,黄芩苷低、中、高剂量(4、 8、 12 mg·kg^(-1))组(均n=10)。采用慢性不可预见性刺激诱导小鼠抑郁症模型,空白对照组小鼠不制模,制模同时各组灌胃给药,每日1次,共21 d。通过检测糖水偏爱分数和强迫游泳总不动时间观察小鼠行为学变化。取脑组织切片,原位末端标记染色(TUNEL)法观察海马细胞凋亡情况,免疫组化法检测海马细胞中calbindin-D28K表达水平。结果与空白对照组比较,模型组小鼠糖水偏爱分数显著降低(P <0.01),强迫游泳总不动时间显著增加(P <0.01),细胞凋亡数量显著增多(P <0.01), calbindin-D28K表达显著减少(P <0.01)。与模型组比较,黄芩苷低、中、高剂量组和阳性对照组小鼠糖水偏爱分数显著增加(P <0.01),强迫游泳总不动时间显著减少(P <0.01),细胞凋亡数量明显减少(P <0.01),海马细胞calbindin-D28K的表达增多(P <0.01);且黄芩苷高剂量组与阳性对照组各指标相当(P> 0.05)。结论黄芩苷可改善抑郁症模型小鼠行为,减少海马神经细胞凋亡,诱导海马calbindin-D28K表达。

AIM To investigate the effects of baicalin on behavioral changes and calbindin-D28 K expression in hippocampus of depression model mice.METHODS Sixty healthy adult ICR mice were randomly divided into blank control group,model group,positive control(fluoxetine 5.2 mg·kg-1) group,and baicalin4,8,12 mg·kg-1 groups(n = 10).The depression model was induced by chronic unpredictable stimulation.The mice in the blank control group were not treated.Each group was intragastrically administered once a day for21 days.The behavioral changes of the mice were observed by detecting the sugar water preference score and the total immobility time of forced swimming.The brain tissue sections were taken and the apoptosis of hippocampus cells was observed by TUNEL.The expression of calbindin-D28 K in hippocampus was detected by immunohistochemistry.RESULTS Compared with the blank control group,the sugar water preference score of the model group was significantly decreased(P < 0.01),the total immobility time of forced swimming was significantly increased(P < 0.01),and the number of apoptosis was significantly increased(P < 0.01),the expression of calbindin-D28 K was significantly decreased(P < 0.01).Compared with the model group,the sugar water preference scores of baicalin 4,8,12 mg·kg-1 groups and positive control group were significantly increased(P < 0.01),the total immobility time of forced swimming was significantly reduced(P < 0.01),the number of apoptosis was significantly decreased(P < 0.01),and the expression of calbindin-D28 K in hippocampus was increased(P < 0.01).And the index in the baicalin 12 mg·kg-1 group was similar with the positive control group(P > 0.05).CONCLUSION Baicalin can improve the behavior of depression model mice,reduce the apoptosis of hippocampus and induce the expression of calbindin-D28 K.