模拟手工搬举作业局部肌肉疲劳相关代谢和炎性标志物研究

Research on metabolic and inflammatory biomarkers related to local muscle fatigue in workers with simulated manual lifting

目的探讨中等负荷重复性手工搬举作业致局部肌肉疲劳作业者血生化指标与局部肌肉疲劳间的关系。方法选取5名健康男性志愿者为研究对象,对其进行重复性模拟手工搬举作业,搬举4个时段(T1～T4),每个时段10 min,每个时段后暂停3 min,对其局部肌肉进行主观疲劳感觉(RPE)评分,同时采集肘静脉血检测血清钙离子、肌酸激酶、软骨寡聚基质蛋白(COMP)、氨、乳酸、乳酸脱氢酶(LDH)、C-反应蛋白(CRP)、Ⅱ型胶原质C末端端肽(CTX-Ⅱ)指标。结果研究对象RPE得分和血清肌酸激酶水平均随搬举时间的增加而增加(P<0.05)。研究对象在T2、T3、T4时段血清钙离子水平均高于T1时段(P<0.05);T3、T4时段血清COMP水平均高于T1时段(P<0.05)。研究对象各搬举时段血清中氨、乳酸、LDH、CRP和CTX-Ⅱ分别比较,差异均无统计学意义(P>0.05)。血清中肌酸激酶、钙离子和LDH水平均与RPE评分均呈正相关(相关系数分别为0.840、0.512和0.741,P<0.01)。结论血清肌酸激酶是反映肌肉疲劳的灵敏、有效、客观指标,适合评价中等负荷重复性活动的疲劳进程;血清钙离子与肌肉疲劳有良好相关性,血清COMP在一定程度上可反映肌肉疲劳。

Objective To analyze the relationship between the blood biochemical indexes and the local muscle fatigue of operators with local muscle fatigue caused by moderate-load repetitive manual lifting.Methods Five healthy male volunteers were selected as the research subjects.They repeatedly performed simulated manual lifting operation for four periods(T1-T4),10 minutes per period.Each period was suspended for 3 minutes to be accessed for the rating of perceived exertion(RPE)score of local muscle.Meanwhile,their venous blood was collected to be detected for serum calcium ion,creatine kinase,cartilage oligomeric matrix protein(COMP),ammonia,lactic acid,lactate dehydrogenase(LDH),C-reactive protein(CRP),and collagen typeⅡC terminal telopeptide(CTX-Ⅱ).Results The RPE score and serum creatine kinase level of the subjects increased with the increasing of lifting time(P<0.05).Serum calcium ion levels in time periods T2,T3 and T4 were higher than that in T1(P<0.05).Serum COMP levels in T3 and T4 were higher than that in T1(P<0.05).The levels of ammonia,lactic acid,LDH,CRP and CTX-Ⅱin each time period showed no statistical significance(P>0.05).Serum creatine kinase,calcium ion and LDH levels were positively correlated with RPE score(correlation coefficient were 0.840,0.512,0.741,respectively,P<0.01).Conclusion Serum creatine kinase is a sensitive,effective and objective index reflecting muscle fatigue,which is suitable for evaluating the fatigue process of moderate-load repetitive activities.Serum calcium ion has a good correlation with muscle fatigue,and serum COMP can reflect muscle fatigue to some extent.