甲基强地松龙对颅脑损伤后脑水肿及脂质过氧化反应的影响

Effects of methylprednisolone on cerebral edema and lipid peroxidation in rats after brain trauma

目的观察甲基强的松龙对大鼠颅脑损伤后脑水肿及脂质过氧化反应的影响,并探讨其作用机制。方法将75只SD大鼠随机分为3组:治疗组(35只)、对照组(35只)、正常组(5只),均采用骨窗形成后硬膜外打击法造成鼠脑挫裂伤。正常组麻醉后,只行开颅手术,不作头颅打击,治疗组大鼠致伤后即刻腹腔内注射50 mg/kg甲基强的松龙,对照组则注射50 mg/kg生理盐水。对照组和治疗组大鼠分别在伤后6、12、24、48、72、96、120 h断头取脑,观察各组各时点血肿周围脑组织超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果对照组各时点SOD活性明显低于正常组,而MDA含量明显高于正常组,对照组各时间点含水量与SOD活力呈明显负相关,与MDA含量呈明显正相关。实验组SOD活力显著高于对照组,12~96 h时间点MDA明显低于对照组,实验组含水量与SOD活力呈显著负相关,与MDA呈显著正相关。结论甲基强的松龙使脑出血氧化损害明显减轻,激素可能是治疗脑出血后脑水肿的一个重要途径。

Objective To observe the role of methylprednisolone on cerebral edema and lipid peroxidation in rats after brain trauma.Methods 75 rats were divided into three groups: treatment group(35 rats extradurally hit from the bone window and then treated with methylprednisolone 30 mg/kg),control group(35 rats treated with physiologic saline after hitting)and normal group(only treated by craniotomy without hitting and injection).The activity of SOD and the contents of MDA were detected at diferent points of time after hitting.Results Compared with the normal group,the activity of SOD in control group was obviously decreased while the contents of MDA was significantly increased.Water contents were obviously positively correlated with MDA,while they were significant nagtively correlated with SOD.Compared with the control group,in the treatment group MDA at 12～96 h was obviously lower and SOD was higher.Water contents were positively correlated with MDA,while they were negatively correlated with SOD.Conclusion Treatment with methylprednisolone in a rat model of brain trauma obviously reduced ICH oxidative damage.The new hormonic drug may make an important approach to treating brain injury.