急性高原低氧对于大鼠血红蛋白和血浆miR-144表达影响的研究

Effect of acute hypobaric hypoxia at high altitude on the expression of hemoglobin and miR-144 in plasma in rats

目的探讨急性高原低氧对于大鼠血红蛋白和血浆miR-144表达的影响。方法应用低压氧舱构建高原低氧大鼠模型,分为高原低氧暴露3、7、14、28 d实验组以及常压常氧对照组。检测各组大鼠血常规和血浆miR-144表达情况。结果与对照组比较,低压低氧组大鼠血浆miR-144、红细胞计数、血红蛋白含量均随高原低氧时间延长而显著升高(P <0. 05)。Spearman相关性分析提示血浆miR-144与红细胞计数、血红蛋白含量呈正相关。结论急性高原低氧可使大鼠血红蛋白和血浆miR-144表达升高。血浆miR-144可能参与调控高原低氧相关的红系变化。

Objective This study investigated the effect of acute hypobaric hypoxia at high altitude on the expression of hemoglobin and miR-144 in plasma in rats. Methods Adult male Sprague-Dawley rats were randomly assigned to a normoxic group and four hypoxic groups. Rats in hypoxic groups were exposed to simulated hypobaric hypoxia chamber equivalent to 7 000 meters for different durations( 3,7,14 and 28 days). Subsequently,blood routine test and the expression of miR-144 in plasma were measured in each group. Results Compared with the normoxic group,the expression of miR-144,erythrocyte count and hemoglobin in four hypoxic groups increased significantly( P < 0. 05). Spearman correlation analysis showed that the expression of miR-144 in plasma was positively correlated with erythrocyte count and hemoglobin.Conclusion Acute hypobaric hypoxia at high altitude increased the expression of miR-144 in plasma and hemoglobin. It is predicted that miR-144 in plasma could induce polycythemia related to hypobaric hypoxia at high altitude. Regulations on miR-144 expression had a great value in clinical application.