乙肝DNA聚合酶在187例乙型肝炎病毒感染的各类肝病中的检测

An Assay of HBV DNA Polymerase in 187 Cases of HBV Infection

采用免疫沉淀法对187例乙肝病毒感染患者作了DNA聚合酶(DNA--P)的测定。在各类肝炎中DNA—P的阳性检出率以慢活肝最高为66.6％,依次为急性肝炎45％,携带者33.5％,慢迁肝31.5％。本文分析了乙肝的抗原抗体系统与DNA—P检出率的关系。经统计学处理,DNA—P检出率与HBsAg滴度增高呈正相关γs=0.99,P<0.01。HBeAg阳性组DNA—P检出率为62.4％,抗—HBe阳性组DNA—P检出率为29.5％,抗—HBs阳性8例、DNA—P均为阴性。抗—HBc—IgM强阳性组DNA—P检出率为86.4％。作者认为1/3无症状携带者DNA—P活力增高表明病毒在复制,因此携带者在流行病学上的意义不可忽视。又鉴于18例抗—HBe阳性血清DNA—P活力增高,因此在判断HBeAg^-/抗—HBe^+患者病毒复制情况时,应予慎重。

Enzyme activity of HBV DNA polymerase ( DNA-P ) in 187 cases with HBV infection was assayed with immune sedimentation method. The percentages of DNA-P positive cases in different types of HBV infection were found in decreasing order as follow: chronic active hepatitis ( 66.6% ) , acute hepatitis ( 45% ) , asymptomatic carriers (33.5%) and chronic persistent hepatitis (31.5%). The relation between antigenantibody system of hepatitis B and DNA-P positive reaction was analysed statistically, showing that DNA-P positive correlated with high titers of HBsAg (γs = 0.99,P<0.01); 62.4% of HBeAg positive group and 29.5% of anti-HBe positive group were DNA-P positive; all of the 8 anti-HBs positive cases were DNA-P negative; and 86.4% of strong anti-HBc-IgM positive group were DNA-P positive.Since the high enzyme activity of HBV DNA-P, which always indicates viral replication, was seen in about one-third of the asymptomatic carriers, the authors emphasize the epidemiological significance of virus carriers. In addition, as high enzyme activity was seen in 18 anti-HBe positive sera, care should be taken in estimating whether viral replication is present or not in HBeAg-/anti-HBe+ patients.