摘要
目的 建立RP HPLC测定当归四逆颗粒中阿魏酸的定量方法。方法 以反相高效液相法测定阿魏酸的含量。固定相 :ShimpackCLC_ODS(150mm× 6mm) ;流动相 :含 0 2 %甲醇的乙腈 含 0 1%磷酸和 0 1%三乙胺水溶液 (2 0∶80 ) ;检测波长 3 2 4nm。结果 阿魏酸在 1 2 4～ 2 4 80 μg/ml范围内线性良好 (r =0 9998) ,平均加样回收率为 98 7% ,RSD为 1 7%。 结论 定量方法简便 ,准确 。
Objective To develop a method for det ermining the content of ferulic acid in Dangguisini granules Method The ferulic acid i n Dangguisini granules was determined by HPLC The separation was performed on S himpack CLC_ODS column with acetonitrile(containing 0 2% methanol)-0 1% trie t hylamine(containing 0 1% phosphoric acid(20∶80) as mobile phase, and detected at 324 nm Results The calibration curve was linear (r=0 9998) in the range of 1 24～24 80 μg/ml for ferulic acid The av erage recovery was 98 67% and RSD was 1 7% Conclusion The method was simpl e, specific and accurate,and can effectively control the quality of Dangguisini g ranules
出处
《江苏药学与临床研究》
2003年第6期21-23,共3页
Jiangsu Pharmacertical and Clinical Research
