6-疏基嘌呤,硫唑嘌呤,8-氮杂鸟嘌呤的滤纸基质室温燐光法研究

The Paper Substrate Room Temperature Phosphorescence of 6-Mercapto Purine, Azathiopurine, 8-Azaguanine

建立了 6 巯基嘌呤、硫唑嘌呤和 8 氮杂鸟嘌呤 3种抗癌嘌呤化合物的滤纸基质室温光分析新方法。详细探讨了影响其室温光发射的各种因素。该方法的线性范围、检出限、精密度分别为 6 巯基嘌呤2 0～ 4 .0× 10 2 μmol/L ,1.3μmol/L ,4 .1% ;硫唑嘌呤 2 .0～ 6 .0× 10 2 μmol/L、1.2 μmol/L、3.8% ;8 氮杂鸟嘌呤1.1～ 2 .2× 10 2 μmol/L、7.4× 10 -2 μmol/L、1.3%。用该方法进行尿液中嘌呤化合物的回收测定 ,回收率为 96 .2 %～ 10 1.7% ;进行 6 MP在药片中的回收测定 ,回收率为 96 .2 %～ 10 2 .1%。

Paper substrate room temperature phosphorescence (PS-RTP) of 6-Mercaptopurine (6-MP), Azathiopurine(BAN), and 8-Azaguanine (8-Azan) was investigated. Various factors affecting the SS-RTP signal were discussed in detail. The corresponding analytical methods were established. The linear dynamic range (LDR) was 2.0～4.0×10 2 μmol/L for 6-MP, 2.0～6.0×10 2 μmol/L for BAN, 1.1～2.2×10 2 μmol/L for 8-Azan, respectively. Limit of detection (LOD) was 1.3 μmol/L for 6-MP, 1.2 μmol/L for BAN, 7.4×10 -2 μmol/L for 8-Azan, respectively. The relative standard deviation was 4.1% for 6-MP, 3.8% for BAN, 1.3% for 8-Azan. Average recoveries for the three purine compounds in urine sample were 96.2%～101 7%, respectively. And the recovery of 6-MP added to pharmaceutical preparation was 96.2%～102.14%.