龙竹的组织培养

Tissue Culture of Dendrocalamus giganteus

以龙竹的幼枝节段作为接种外植体,采用从腋芽-丛生芽-完整植株的繁殖途径进行繁殖。结果表明:在MS+BA 2 mg·L-1+NAA 0.2 mg·L-1+PVP 250 mg·L-1+蔗糖30 g·L-1培养基上培养10-20 d可诱导其腋芽萌发,新芽接种于MS附加BA 2 mg·L-1+KT 0.5 mg·L-1+CW 100 mL·L-1+蔗糖30 g·L-1的培养基上培养20 d可诱导形成丛生芽,丛生芽在继代培养过程中每20-25d可增殖3-5倍。把丛生芽接种于1/2MS+NAA2mL·L-1+IAA2mL·L-1+蔗糖20 g·L-1培养基上培养4周后可诱导形成完整的根系,小植株移栽成活率可达98％。该体系的建立为龙竹的工厂化育苗奠定了坚实的基础。

The nodal explants of Dendrocalamus giganteus were inoculated on MS medium containing 2 mg/L BA, 0.2 mg/L NAA, 250 mg/L PVP and 30 g/L sucrose for inducing shoots. After being cultured for 20 days, the shoots were bourgeoned. They were then transferred onto the MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT, 100 mL/L CW and 30 g/L sucrose for inducing clustered buds. After 20 days, the clustered buds were formed. The clustered buds was enlarged 3-5 times in an about month. After the clustered buds were cultured on the 1/2 MS medium containing 2 mg/L NAA, 2 mg/L IAA and 20 g/L sucrose for about 30 days, the roots were developed. About 98 % of plantlets survived when they were transferred onto soil. This cultural system will be a solid foundation for commercial proliferation of Dendrocalamus giganteus.