摘要
目的 采用HPLC法测定复方磺胺甲唑混悬剂中磺胺甲唑 (SMZ)和甲氧苄啶 (TMP)的含量。方法 采用Zor baxSB C18色谱柱 ;以磷酸盐缓冲液 乙腈 (75∶2 5)为流动相 ;流量为 1 0ml/min ;检测波长为 2 3 0nm ;用外标法测定。结果 平均回收率 :SMZ为 99 68% ,RSD为 0 2 6% ;TMP为 10 0 6% ,RSD为 0 76%。结论 该方法简便、准确、可靠 ,适用于复方磺胺甲唑混悬剂的定量检测。
Objective To determine SMZ and TMP in Suspension Sulfam ethoxazole Composite by HPLC Method A Zorbax SB-C 18 c olumn was used The mobile phase was phosphoric buffer(pH 2 5,0 025 mol/L)-acet onit rile (75:25) and the flow rate was 1 0 ml/min The UV detection wavelength was 2 30nm Result The average recovey was 99 7%(RSD=0 26 %)and 100 6%(RSD=0 76%)for SMZ and TMP Conclusion: The met hod is sim ple, accurate and reliable The method was suitable for the determination of Su spension Sulfamethoxazole Composite
出处
《江苏药学与临床研究》
2003年第6期28-30,共3页
Jiangsu Pharmacertical and Clinical Research
