聚合酶链反应在慢性乙型肝炎病毒感染者中的实用意义

Practical Significance of Polymerase Chain Reaction in Chronic Hepatitis B Viral Infection

应用聚合酶链反应检测51例慢性肝炎,19例慢性HBsAg携带者(ASC)和26例HBV感染后的健康者血清中的HBV DNA。结果14例HBeAg阳性患者HBV DNA全部阳性,37例HBeAg阴性者中24例阳性(64.9％)。其中HBV·M阴性9例,有6例阳性。5例抗-HBs阳性者3例阳性。ASC和健康者PCR-HBV DNA检出率分别为36.8％和26.9％,明显低于慢性肝炎的74.5％。结果提示,慢性肝炎患者HBeAg阴性甚至HBV·M阴性时,往往大部分仍有HBV复制,且可能与病变活动有关。ASC和健康者总体病毒复制水平较低,可能与无明显的肝脏病表现有关。

Serum HBV DNA was detected in 51 cases of chronic hepatitis, 19 cases of HBsAg carriers and 26 healthy subjects (all after HBV infection) by polymerase chain reaction. Thirty-eight out of 51 patients with chronic hepatitis showed positive PCREB HBV DNA (74.5%).The percentages of positive serum HBV DNA were 100% in HBeAg positive serum CAH, and the 64.9% in 37 cases with HBeAg negative CH. However, HBV DNA was also positive in 6 of 9 cases without any HBV markers and in three of the five with positive anti-HBs. The positive rate of viral DNA was 36.8%in HBsAg carriers and 26.9% in heathy subjects. The positive rates were lower thanthat in cases of chronic hepatitis (P<0.005). The results suggestea that viral repli-cation was still present in the most of patients with chronic hepatitis (includingthose with HBeAg negative and those without any HBV markers). The total level ofviral replication in the serum of HBsAg carriers and healthy subjects was lower thanthat of CH.