摘要
目的:探讨血小板源性生长因子-B(PDGF)与白介素-1α(IL-1α)对动脉平滑肌细胞基质金属蛋白酶(MMP)-2、9及基质金属蛋白酶的组织抑制因子(TIMP)-1表达的影响。方法:通过免疫组化与RT-PCR方法,研究体外原代培养的大鼠主动脉平滑肌细胞在使用PDGF-B及IL-1α的情况下,MMP-2、9及TIMP-1表达的情况。结果:正常大鼠动脉平滑肌细胞有MMP-2与TIMP-1的表达,但没有MMP-9的表达,单独使用PDGF-B与IL-1α不能诱导MMP-9的表达,但是同时使用即可诱导其表达。结论:在病理情况下,PDCF-B与IL-1α协同作用诱导MMP-9表达,降解胶原蛋白,这可能是颅内动脉瘤形成机制中的重要途径之一。
Aim:To study the effects of PDGF-B and IL-1α on the expression of MMP-2,9 and TIMP-1 in the cultured aorta smooth muscle cells of rats. Methods: The smooth muscle cells of rat aorta artery were primarily cultured and the effects of PDGF-B and IL-1α on the expression of MMP-2,9 and TIMP-1 were determined by the immunohistocliemistry and RT-PCR.Results:The aorta smooth muscle cells can secrete MMP-2 and TIMP-1 without PDGF-B and IL-1α,but no MMP-9. Neither PDGF-B nor IL-1α alone induces the expression of MMP-9 in the aorta smooth muscle cells , however, PDGF-B can induce the expression of MMP-9 synergistically with IL-1α. Conclusion: PDGF-B is able to induce the expression of MMP-9 synergistically with IL-1α for degradation of the extracellular matrix on the arterial wall, this maybe one possible mechanism on the pathogenesis of intracranial aneurysms.
出处
《中国临床神经科学》
2003年第4期363-366,共4页
Chinese Journal of Clinical Neurosciences
