仙草多糖的分离纯化及鉴定

STUDY ON MESONA CHINENSIS BENTH POLYSACCHARIDE Ⅰ. ISOLATION, PURIFICATION AND IDENTIFICATION

仙草（Mesona Chiliensis Benth）是我国的一种民间草药。用0.5％NaHCO3抽提,乙醇沉淀得到了仙草多糖的粗品。经过H2O2脱色处理,Sephadex G-75柱层析和硫酸—苯酚法收集单一峰部分,得到仙草多糖纯品（简称MCPS）,薄层层析、凝胶电泳和醋纤薄膜电泳证明其为均一性多糖。红外光谱扫描表明,它具有典型的多糖吸收峰。HPLC法测得相对分子量为4.3×104,经薄层层析确定MCPS的单糖组成为葡萄糖、半乳糖、阿拉伯糖、木糖、鼠李糖,半乳糖醛酸及一种未知单糖。药理实验表明,它具有免疫促进作用与抑瘤效应。

Mesona Chinensis Benth is traditional chinese herbal medicine. The crude polysaccharide can be obtained from Mesona Chinensis Benth by extraction with 0.5% NaHCO_3 and by fraction precipitation with alcohol. It was deeoloured with H_2O_2 and further purification by chromatography on a Sephadex G-75 column. The pure polysaecharide is given the name of Me sona Chinensis Benth polysaccharide (MCPS) which is homogeneous by the analysises of thin-layer chormatogrpahy, agarose gel electrophoresis and cellulose acetate film electrophoresis The analysis of MCPS by IR spectrum scanning gave a special absorption band of polysaccharide. Its molecular weight was estimated to be 43,000 by HPLC and its monosaceharide composition was identified by thin-layer chromatography. The results indecated that MCPS consists of D-glucose, D-galaetose, L-arabinose, xylose, rhamnose galaeturonie acid and an unknown sugar. Preliminary results of pharmacology experiment showed that MCPS have function of increasing immunity and antieaneer effect.