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Rudimentary study on humanization of porcine red blood cells: Enzymatic removal of galactose-α1,3- galactose antigen from porcine red blood cell 被引量:2

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摘要 The serological and biochemical characteriza-tion of porcine red blood cells (pRBCs) are similar to human red blood cells. Porcine erythrocytes are considered as an alternative source for human blood transfusion. But there exist galactose-?,3-galactose antigens (Gal?,3Gal?, 4GalNAcR, abbreviated 酖al antigen) on pRBCs, which can induce anti-aGal antibodies in human serum. The aGal epitopes are the major antigen responsible for hyperacute rejection in xenotransfusion. In this study, recombined soy-bean -galactosidase (rS?GalE) was used to remove the aGal antigens from pPRCs for humanization. The results showed that aGal antigen was cleared by rS?GalE and the structure and function of rS?GalE treated pRBC were normal. The serological and biochemical characteriza-tion of porcine red blood cells (pRBCs) are similar to human red blood cells. Porcine erythrocytes are considered as an alternative source for human blood transfusion. But there exist galactose-?,3-galactose antigens (Gal?,3Gal?, 4GalNAcR, abbreviated 酖al antigen) on pRBCs, which can induce anti-aGal antibodies in human serum. The aGal epitopes are the major antigen responsible for hyperacute rejection in xenotransfusion. In this study, recombined soy-bean -galactosidase (rS?GalE) was used to remove the aGal antigens from pPRCs for humanization. The results showed that aGal antigen was cleared by rS?GalE and the structure and function of rS?GalE treated pRBC were normal.
出处 《Chinese Science Bulletin》 SCIE CAS 2003年第4期343-345,共3页
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  • 1Irma F. Herder,Ana M. Mateo Rosell,Caren M. Zuilen,Peter J. Punt,Cees A. M. J. J. Hondel. Cloning and expression of a member of the Aspergillus niger gene family encoding α-galactosidase[J] 1992,MGG Molecular & General Genetics(3):404~410

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