摘要
Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice Xiushui? The elicitor from the cell wall of M. grisea race 98-186-1G1, termed IE, strongly in-duced the HR response in suspension cultures of rice Xiushui? including increased PAL activity, transcription of pal , pr1, chi, cell death and the generation of NO. The elici-tor prepared from the cell wall of M. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by IE in Xiushui?rice. The increased PAL activity and transcription of pr1, pal, chi genes induced by IE were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription of pr1, pal and chi genes. These data implicated that NO acted as a signal mediating the HR induced by IE in rice and showed that NO, in combination with H2O2, is necessary for induc-tion of cell death by IE in rice suspension cells.
Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice Xiushui? The elicitor from the cell wall of M. grisea race 98-186-1G1, termed IE, strongly in-duced the HR response in suspension cultures of rice Xiushui? including increased PAL activity, transcription of pal , pr1, chi, cell death and the generation of NO. The elici-tor prepared from the cell wall of M. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by IE in Xiushui?rice. The increased PAL activity and transcription of pr1, pal, chi genes induced by IE were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription of pr1, pal and chi genes. These data implicated that NO acted as a signal mediating the HR induced by IE in rice and showed that NO, in combination with H2O2, is necessary for induc-tion of cell death by IE in rice suspension cells.
基金
supported by the National Natural Science Foundation of China(Grant No.39870050).
