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Fine mapping of the Ht2(Helminthosporium turcicum resistance 2) gene in maize 被引量:3

Fine mapping of the Ht2(Helminthosporium turcicum resistance 2) gene in maize
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摘要 Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene, gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding. An F2 mapping population was developed from a cross between a resistant inbred line 77Ht2 and a susceptible inbred line Huobai. With the aid of RFLP marker analyses, the Ht2 gene was mapped between the RFLP markers UMC89 and BNL2.369 on chromosome 8, with a genetic distance of 0.9 cM to BNL2.369. There was a linkage between SSR markers UMC1202, BNLG1152, UMC1149 and the Ht2 gene by SSR assay. Among the SSR markers, the genetic distance between UMC1149 and the Ht2 gene was 7.2 cM. By bulked segregant analysis 7 RAPD-amplified products which were probably linked to the Ht2 gene were selected after screening 450 RAPD primers and converted the single-copy ones into SCAR markers. Linkage analysis showed that the genetic distance between the SCAR marker SD-06633 and the Ht2 gene was 0.4 cM. From these results, a part of linkage map around the Ht2 gene was constructed. Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene, gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding. An F2 mapping population was developed from a cross between a resistant inbred line 77Ht2 and a susceptible inbred line Huobai. With the aid of RFLP marker analyses, the Ht2 gene was mapped between the RFLP markers UMC89 and BNL2.369 on chromosome 8, with a genetic distance of 0.9 cM to BNL2.369. There was a linkage between SSR markers UMC1202, BNLG1152, UMC1149 and the Ht2 gene by SSR assay. Among the SSR markers, the genetic distance between UMC1149 and the Ht2 gene was 7.2 cM. By bulked segregant analysis 7 RAPD-amplified products which were probably linked to the Ht2 gene were selected after screening 450 RAPD primers and converted the single-copy ones into SCAR markers. Linkage analysis showed that the genetic distance between the SCAR marker SD-06633 and the Ht2 gene was 0.4 cM. From these results, a part of linkage map around the Ht2 gene was constructed.
出处 《Chinese Science Bulletin》 SCIE EI CAS 2003年第2期165-169,共5页
基金 This work was jointly supported by the National Transgenic Plant Research Foundation of China (Grant No. J00-A-002) and the National Research Foundation for Key Techniques (The Explo-ration and Application of Crop Resources,2001-2003,04-012) of China.
关键词 玉米 遗传图 分子标记 长蠕孢属 抗枯萎病基因 Ht2 精细定位 maize, Helminthosporium turcicum resistance gene Ht2, fine mapping, genetic map, molecular markers.
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同被引文献36

  • 1佟圣辉,陈刚,王孝杰,王作英,陈丽,岳辉.我国玉米杂优群对主要病害的抗性鉴定与评价[J].杂粮作物,2005,25(2):101-103. 被引量:7
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