双歧杆菌对实验性大肠癌丝裂素活化蛋白激酶和激活蛋白-1的影响

Effect of bifidobacteria on MAPK and AP-1 of experimental large bowel carcinoma

目的 探索青春型双歧杆菌的体内抑瘤机制。方法 以激光共聚焦显微镜检测了大肠癌裸鼠移植瘤细胞外信号调节蛋白激酶(ERK)1/2、c-jun氨基末端激酶(JNK)和p38以及激活蛋白-1(AP-1)的主要成份 c-fos和c-jun的含量。结果 大肠癌裸鼠移植瘤经双歧杆菌处理后,其ERK1/2、JNK、c-fos和c-jun的平均荧光强度均显著低于肿瘤对照组(P<0.01),而p38的平均荧光强度在两组间差异无显著性(P>0.05)。结论 青春型双歧杆菌体内能明显降低大肠癌ERK1/2和JNK的活性以及c-fos和c-jun的表达。

Objective To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. Methods The content of extracellular signal regulated protein (ERK) 1/2, C-Jun N-terminal kinase (JNK), p38, c-fos and c-jun of nude mouse transplantation tumors of large bowel carcinoma was detected by using laser scanning confocal microscopy. C-fos and c-jun were the main composition of activator pro- tein 1. Results After the nude mouse transplantation tumors of large bowel carcinoma were treated with bifidobacteria, the average fluorescent intensity of ERK1/2, JNK, c-fos and c-jun was significantly lower than that in tumor control group (P < 0. 01). There was no significant difference in the average flures- cent intensity of p38 between two groups (P > 0. 05). Conclusion Bifidobacteria adolescence could markedly decrease the activity of ERK1/2, JNK and the expression of c-fos, c-jun.